CAJ | 학술논문

　　为了对鸟类性别分化中起重要作用的基因进行深入分析，对家鸡性反转胚胎雌激素及相关调控基因表达模式进行了检测，分析这些性激素在鸟类性别分化调控起到的作用。选择 ER、3βHSD、P450c17、P450arom 4个基因为主要研究对象，采用外源注入formestane（福美司坦）和17β-estradiol（17β雌二醇）2种手段，并设置雌雄鸡胚2个对照组，检测以上基因在胚胎早期发育过程中(84、96、120、144、168、192 h)的表达变化趋势。ER、3βHSD、P450c17、P450arom 基因的表达变化在注入外源激素的试验组同未注射组间，差异均显著(P＜0.05)或极显著(P＜0.01)，其中ER表现为下降趋势，P450arom表达激增，3βHSD、P450c17基因表达变化缺乏明显规律。雌激素调控是一个复杂的过程，在外源激素注入过程后，原有调控模式可能会被打乱。
　　위료대조류성별분화중기중요작용적기인진행심입분석，대가계성반전배태자격소급상관조공기인표체모식진행료검측，분석저사성격소재조류성별분화조공기도적작용。선택 ER、3βHSD、P450c17、P450arom 4개기인위주요연구대상，채용외원주입formestane（복미사탄）화17β-estradiol（17β자이순）2충수단，병설치자웅계배2개대조조，검측이상기인재배태조기발육과정중(84、96、120、144、168、192 h)적표체변화추세。ER、3βHSD、P450c17、P450arom 기인적표체변화재주입외원격소적시험조동미주사조간，차이균현저(P＜0.05)혹겁현저(P＜0.01)，기중ER표현위하강추세，P450arom표체격증，3βHSD、P450c17기인표체변화결핍명현규률。자격소조공시일개복잡적과정，재외원격소주입과정후，원유조공모식가능회피타란。
In order to analyze the genes that played an important role in sex differentiation of birds, this study was designed to detect expression profiles of estrogens and its related genes in sex-reversed chicken embryos. In the present study, after injection with formestane and 17β-estradiol, expression of ER, 3βHSD, P450c17, and p450arom were measured at the early development stages (84, 96, 120, 144, 168、192 h incubation) in chicken embryos by Real-time PCR. Embryos treated with formestane and 17β-estradiol were designed as test group, while embryos non-treated but sex differential comprised control group. The results showed that:expression of ER, 3βHSD, P450c17 and P450arom in control group showed normal expression trend, but when compared with test group, the difference was significant (P<0.05 or P<0.01). For the test group, ER expression showed a downward trend, whereas, p450 arom expression increased sharply. However, there was no obvious trend for the expression trend of 3βHSD and P450c17 genes. It was indicated that estrogen regulation was a complex process. Once injected with exogenous hormone, the normal regulation process would be disturbed significantly.