CAJ | 학술논문

　　为了获得高密度培养的解磷细菌菌剂，以一株解磷巨大芽孢杆菌为研究对象，以OD600为依据测定活菌数量，优化最佳液体发酵培养条件。首先采用单因素试验对其培养条件如接种量、装液量、发酵温度、起始pH、发酵时间、摇床转速等进行筛选，获得单因素试验最佳值；在此基础上，利用L9(34)正交试验对其培养条件进行优化及验证，并制作菌株生长曲线。研究结果表明：该株巨大芽孢杆菌的最佳培养条件为：发酵温度30℃、起始pH 8.0、装液量20 mL/250 mL三角瓶、接种量3%、摇床转速250 r/min、培养时间22 h。在此最优条件下培养，以平板涂布法计数，发酵液最终活菌数达到3.2×109 cfu/mL以上。培养2~8 h，菌体生长处于对数期，8 h后菌体生长进入稳定期，22 h后进入衰亡期。试验结果为工业生产解磷巨大芽孢杆菌菌剂提供了基础数据。
　　위료획득고밀도배양적해린세균균제，이일주해린거대아포간균위연구대상，이OD600위의거측정활균수량，우화최가액체발효배양조건。수선채용단인소시험대기배양조건여접충량、장액량、발효온도、기시pH、발효시간、요상전속등진행사선，획득단인소시험최가치；재차기출상，이용L9(34)정교시험대기배양조건진행우화급험증，병제작균주생장곡선。연구결과표명：해주거대아포간균적최가배양조건위：발효온도30℃、기시pH 8.0、장액량20 mL/250 mL삼각병、접충량3%、요상전속250 r/min、배양시간22 h。재차최우조건하배양，이평판도포법계수，발효액최종활균수체도3.2×109 cfu/mL이상。배양2~8 h，균체생장처우대수기，8 h후균체생장진입은정기，22 h후진입쇠망기。시험결과위공업생산해린거대아포간균균제제공료기출수거。
To obtain high density bacterial fertilizer of phosphate-solubilizing bacteria, culture conditions for a strain of phosphate-solubilizing bacteria Bacillus megaterium isolated from soil were optimized, and OD600 of the broth was used as a standard to judge the number of viable cells. Firstly, single factor experiments were used to research the effects of different factors of inoculation amount, medium volume, culture temperature, initial pH, incubation time, and rotate speed on submerged fermentation and the best values of single factors were obtained. Then orthogonal experiment of L9(34) was used to optimize the optimal culture conditions. After that, growth curve of the strain was described. The results showed that the optimal culture conditions were:temperature of 30℃, initial pH of 8.0, medium volume of 20 mL/250 mL flask, inoculation amount of 3%, rotate speed of 250 r/min, and incubation time of 22 h; under the optimal culture conditions, the number of viable cells of Bacillus megaterium reached above 3.2×109 cfu/mL. The period between 2 hours to 8 hours after inoculation was logarithmic phase for cell growth, then cell growth was in the stable stage, and after 22 hours, decline phase started. The results could provide basic data for production of phosphate-solubilizing bacterial fertilizer for agricultural application.