CAJ | 학술논문

　　目的探讨CATSPER1蛋白在精子运动中的功能。方法将正常精液标本与终浓度分别为0.8μg/mL、4μg/mL、20μg/mL的CATSPER1抗体混合孵育，然后于37℃1h、2h、6h用计算机辅助分析系统（CASA）进行精液参数分析。结果在不同时间，前向运动精子（a+b级）、快速前向运动精子（a级）的百分比均较对照组明显减少并且成剂量依赖性,慢速前向运动精子（b级）受抗体抑制的作用不明显。结论 CATSPER1在精子运动过程中发挥重要功能，CATSPER1抗体主要抑制精子快速前向运动能力。
　　목적탐토CATSPER1단백재정자운동중적공능。방법장정상정액표본여종농도분별위0.8μg/mL、4μg/mL、20μg/mL적CATSPER1항체혼합부육，연후우37℃1h、2h、6h용계산궤보조분석계통（CASA）진행정액삼수분석。결과재불동시간，전향운동정자（a+b급）、쾌속전향운동정자（a급）적백분비균교대조조명현감소병차성제량의뢰성,만속전향운동정자（b급）수항체억제적작용불명현。결론 CATSPER1재정자운동과정중발휘중요공능，CATSPER1항체주요억제정자쾌속전향운동능력。
?Objective?Objective: To evaluate the function of cation channel of sperm 1 (CATSPER1) protein in human sperm motility; Methods The sperm samples from normozoospermic donors were incucbated with different CATSPER1 antibody at final concentrations of 0,0.8,4,20μg/mL.Sperm motility was measured by means of computer-assisted semen analysis after 1, 2 and 6 hours incubation at 37℃. Results Progressive motility (class a+b) and fast progressive motility (class a) of sperm were both inhibited under the use of CATSPER1 antibody in different concentrations and significant dose-dependent changes were observed (respectively, P<0.01 and P<0.05)and slow motility sperms were not inhibited at different time. Conclusion CATSPER1 protein plays an important role in maintaining the motility of mature sperm of which fast progressive motility can be inhibited by CATSPER1 antibody.