中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
17期
177-181
,共5页
雷强%谢春琼%刘鲁峰%湛方栋%祖艳群%何永美
雷彊%謝春瓊%劉魯峰%湛方棟%祖豔群%何永美
뢰강%사춘경%류로봉%담방동%조염군%하영미
镉%铅锌矿区%根际真菌%培养液%pH值
鎘%鉛鋅礦區%根際真菌%培養液%pH值
력%연자광구%근제진균%배양액%pH치
cadmium%lead-zinc mining area%rhizosphere fungi%liquid medium%pH value
为了明确镉(Cd)胁迫对云南会泽铅锌矿区中华山蓼根际真菌氢离子分泌行为的影响,探讨矿区野生植物根际真菌的生态作用。采用液体培养法,分析不同浓度(0、0.05、0.5、5 mmol/L) Cd处理条件下根际真菌培养液的pH值。结果表明:(1)铅锌矿区中华山蓼根际真菌氢离子分泌能力及其对Cd处理的响应在不同菌株间存在明显差异;(2)没有施加Cd处理,产酸能力强的真菌培养液pH值低至4.02,产酸能力弱的pH值高至8.11;(3)Cd处理对产酸能力强的真菌培养液pH值影响不明显,但导致产酸能力较弱的真菌培养液pH值显著下降,降幅最大达到3.21~3.86,培养液pH值与Cd浓度呈极显著或显著负相关。可见,Cd胁迫显著促进产酸能力较弱的铅锌矿区中华山蓼根际真菌分泌氢离子。
為瞭明確鎘(Cd)脅迫對雲南會澤鉛鋅礦區中華山蓼根際真菌氫離子分泌行為的影響,探討礦區野生植物根際真菌的生態作用。採用液體培養法,分析不同濃度(0、0.05、0.5、5 mmol/L) Cd處理條件下根際真菌培養液的pH值。結果錶明:(1)鉛鋅礦區中華山蓼根際真菌氫離子分泌能力及其對Cd處理的響應在不同菌株間存在明顯差異;(2)沒有施加Cd處理,產痠能力彊的真菌培養液pH值低至4.02,產痠能力弱的pH值高至8.11;(3)Cd處理對產痠能力彊的真菌培養液pH值影響不明顯,但導緻產痠能力較弱的真菌培養液pH值顯著下降,降幅最大達到3.21~3.86,培養液pH值與Cd濃度呈極顯著或顯著負相關。可見,Cd脅迫顯著促進產痠能力較弱的鉛鋅礦區中華山蓼根際真菌分泌氫離子。
위료명학력(Cd)협박대운남회택연자광구중화산료근제진균경리자분비행위적영향,탐토광구야생식물근제진균적생태작용。채용액체배양법,분석불동농도(0、0.05、0.5、5 mmol/L) Cd처리조건하근제진균배양액적pH치。결과표명:(1)연자광구중화산료근제진균경리자분비능력급기대Cd처리적향응재불동균주간존재명현차이;(2)몰유시가Cd처리,산산능력강적진균배양액pH치저지4.02,산산능력약적pH치고지8.11;(3)Cd처리대산산능력강적진균배양액pH치영향불명현,단도치산산능력교약적진균배양액pH치현저하강,강폭최대체도3.21~3.86,배양액pH치여Cd농도정겁현저혹현저부상관。가견,Cd협박현저촉진산산능력교약적연자광구중화산료근제진균분비경리자。
The aims were to elucidate the effects of cadmium (Cd) on the hydrogen ion secretion of the rhizosphere fungi of Oxyria sinensis Hemsle in abandoned lead-zinc mining area in Huize, Yunnan Province, China, and explore the ecological role of wild plants rhizosphere fungi in mining area. The pH value of liquid medium for culturing rhizosphere fungi under Cd treatment with different concentrations (0, 0.05, 0.5, 5 mmol/L) was investigated by liquid culture methods. The results showed that: (1) there were significantly difference on hydrogen ion secretion and its responses to Cd treatment of the rhizosphere fungi between the different strains. (2) Under without Cd treatment, the pH value of liquid media for culture the rhizosphere fungi with great or weak acid producing ability was low to 4.02 or high to 8.11, respectively. (3) Cd treatments had no significant effect on pH value of liquid media for culture the rhizosphere fungi with great acid producing ability, but resulted in pH value decreased significantly of liquid media for culture the rhizosphere fungi with weak acid producing ability. The largest decline was 3.21-3.86. Significant or very significant negative correlation was observed between pH value of liquid media and Cd treatment concentrations. It indicated that the secretion of hydrogen ion by the rhizosphere fungi with weak acid producing ability would be significantly promoted by Cd treatments.