CAJ | 학술논문

　　采用半离体培养法和联苯胺-过氧化氢法研究了‘砀山酥梨’花粉萌发特性和柱头可授性。通过对‘酥梨’的最适授粉时间以及7种授粉处理坐果率的研究，探讨其授粉特性。结果表明：开花后每天酥梨柱头活性最佳的时间是11时至15时，柱头最佳授粉时间为开花后第4~5天；‘酥梨’花粉最适萌发温度为25℃，花粉管生长最有利的温度为22~25℃，在相同温度下，花粉萌发率和花粉管长度随时间增加而增加。7种授粉处理‘酥梨’的花朵坐果率中，没有昆虫授粉的‘酥梨’坐果率为0.48%和0.44%，自然授粉和人工授粉坐果率分别为2.83%和13.05%，‘酥梨’×‘雪花梨’组蜜蜂授粉的坐果率为32.95%，新鲜花粉和商品花粉坐果率分别为70.2%和13.05%。由此可见，‘酥梨’是异花虫媒果树，蜜蜂可以在‘酥梨’柱头活性最高时进行授粉，从而提高‘酥梨’的坐果率，同时通过对‘酥梨’花器官生理活性和坐果习性的研究，为‘酥梨’的授粉和生产提供基础资料。
　　채용반리체배양법화련분알-과양화경법연구료‘탕산소리’화분맹발특성화주두가수성。통과대‘소리’적최괄수분시간이급7충수분처리좌과솔적연구，탐토기수분특성。결과표명：개화후매천소리주두활성최가적시간시11시지15시，주두최가수분시간위개화후제4~5천；‘소리’화분최괄맹발온도위25℃，화분관생장최유리적온도위22~25℃，재상동온도하，화분맹발솔화화분관장도수시간증가이증가。7충수분처리‘소리’적화타좌과솔중，몰유곤충수분적‘소리’좌과솔위0.48%화0.44%，자연수분화인공수분좌과솔분별위2.83%화13.05%，‘소리’×‘설화리’조밀봉수분적좌과솔위32.95%，신선화분화상품화분좌과솔분별위70.2%화13.05%。유차가견，‘소리’시이화충매과수，밀봉가이재‘소리’주두활성최고시진행수분，종이제고‘소리’적좌과솔，동시통과대‘소리’화기관생리활성화좌과습성적연구，위‘소리’적수분화생산제공기출자료。
Using the semi-vitro culture and benzidine-H2O2 methods to study the pollen germination characteristic and stigma receptivity of‘Dangshan suli’, through the research of optimal period of pollination and the fruit set percentage of seven pollination treatments to study pollination characteristic of pear. The results showed that: the optimal time of stigma activity was at 11:00-15:00 in each day after flowering,the optimal period of pollination of the stigma was the 4th to 5th day after flowering. The optimal temperatures of pollen germination and growth of pollen tube were 25℃ and 22-25℃ respectively, the percentage of pollen germination and the length of pollen tube would be increased as time increase at the same temperature. Fruit set percentage of flowers of seven pollination treatments, the fruit set percentage without insects’pollinating was 0.48%-0.44% , fruit set percentage with natural pollination and artificial pollination were 2.83% and 13.05% respectively, fruit-set percentage with bees’pollination for treatment of‘Dangshan suli’versus‘Xuehuali’was 32.95%, and fruit set percentage of the fresh pollen and commodity pollen was 70.2% and 13.05% respectively. It was showed that‘Dangshan suli’was entomophilous and cross-pollinating fruit tree, and bees could pollinate for‘Dangshan suli’to improve the fruit set percentage of it when the stigma activity was in the highest period. Through the research of physiological activity of floral organ and fruit set habit, it also could provide the basic information of pollination and production for‘Dangshan suli’.