中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
16期
63-68
,共6页
李杰%章金辉%朱根发%王再花
李傑%章金輝%硃根髮%王再花
리걸%장금휘%주근발%왕재화
石斛属%种质资源鉴定%指纹图谱%应用
石斛屬%種質資源鑒定%指紋圖譜%應用
석곡속%충질자원감정%지문도보%응용
Dendrobium%germplasm identification%fingerprinting%application
为了鉴别石斛属植物不同种质,区分石斛伪品劣品,笔者综述了传统的形态学与显微鉴定、细胞学、孢粉学以及指纹图谱技术(化学指纹图谱和DNA指纹图谱)的特点,及其在石斛属植物种质资源鉴定中的应用,发现指纹图谱技术对石斛属植物的种源和道地性鉴定以及石斛类药材质量控制十分有效,但指纹图谱标准和评价体系还存在不足,今后还需在野生石斛资源的评价与合理利用、指纹图谱与药理作用相关性、遗传改良和基因克隆等方面开展深入研究。
為瞭鑒彆石斛屬植物不同種質,區分石斛偽品劣品,筆者綜述瞭傳統的形態學與顯微鑒定、細胞學、孢粉學以及指紋圖譜技術(化學指紋圖譜和DNA指紋圖譜)的特點,及其在石斛屬植物種質資源鑒定中的應用,髮現指紋圖譜技術對石斛屬植物的種源和道地性鑒定以及石斛類藥材質量控製十分有效,但指紋圖譜標準和評價體繫還存在不足,今後還需在野生石斛資源的評價與閤理利用、指紋圖譜與藥理作用相關性、遺傳改良和基因剋隆等方麵開展深入研究。
위료감별석곡속식물불동충질,구분석곡위품렬품,필자종술료전통적형태학여현미감정、세포학、포분학이급지문도보기술(화학지문도보화DNA지문도보)적특점,급기재석곡속식물충질자원감정중적응용,발현지문도보기술대석곡속식물적충원화도지성감정이급석곡류약재질량공제십분유효,단지문도보표준화평개체계환존재불족,금후환수재야생석곡자원적평개여합리이용、지문도보여약리작용상관성、유전개량화기인극륭등방면개전심입연구。
In order to identify the germplasm sources, fake and inferior Dendrobium in the market, multiple methods have been applied in identification of Dendrobium germplasm. In the paper, characteristics and applications of the morphological, microstructure, cytological, palynological, chemical fingerprinting and DNA fingerprinting methods were reviewed. It was found that fingerprintings were effective for discrimination the germplasm and geo-herbalism of Dendrobium, and medicinal material quality control, while the criterions and evaluation system of fingerprintings should be further completed, and it was necessary to focus on the aspects of reasonable evaluation and utilization wild resources, combination of fingerprintings and pharmacological action, genetic improvement and gene cloning for the future research in Dendrobium.