CAJ | 학술논문

　　为提高β-甘露聚糖酶产量，探究其发酵生产的规律，以β-甘露聚糖酶高产菌株地衣芽孢杆菌(Bacillus licheniformis)HDYM-04为供试菌株，以初始魔芋粉加入量2%、接种量6.7%、初始pH 8.0、培养温度37℃、搅拌速率300 r/min、通气量3 L/min、发酵周期30 h为基础发酵条件进行5 L发酵罐发酵。基于 Logistic 方程和 Luedeking-Piret 等方程建立了该菌株的菌体生长、产物生成、底物消耗3个分批发酵动力学模型，其中菌体生长动力学模型为：dXdt =0.431X(1- X15.522)、底物消耗动力学模型为- dSdt =1.11dXdt +0.0002dPdt +0.0008X 、产物生成动力学模型为 dPdt =133.1dXdt +222.87X ，其相关系数R2分别为0.99021、0.98908、0.98812，能真实描述菌体发酵过程中菌体生长、酶的合成以及底物消耗的情况。
　　위제고β-감로취당매산량，탐구기발효생산적규률，이β-감로취당매고산균주지의아포간균(Bacillus licheniformis)HDYM-04위공시균주，이초시마우분가입량2%、접충량6.7%、초시pH 8.0、배양온도37℃、교반속솔300 r/min、통기량3 L/min、발효주기30 h위기출발효조건진행5 L발효관발효。기우 Logistic 방정화 Luedeking-Piret 등방정건립료해균주적균체생장、산물생성、저물소모3개분비발효동역학모형，기중균체생장동역학모형위：dXdt =0.431X(1- X15.522)、저물소모동역학모형위- dSdt =1.11dXdt +0.0002dPdt +0.0008X 、산물생성동역학모형위 dPdt =133.1dXdt +222.87X ，기상관계수R2분별위0.99021、0.98908、0.98812，능진실묘술균체발효과정중균체생장、매적합성이급저물소모적정황。
In order to increase the production of β-mannase and research its regular pattern during fermentation process, high β-mannase producing strain, Bacillus licheniformis HDYM-04, was used to investigate the kinetics model based on the optimal fermentation conditions: the strain was fermentated at 37℃for 30 h with agitation speed at 300 r/min and aeration rate at 3 L/min in a 5 L fermantor, the initial addition of konjac flour was 2% (w/v) and the initial pH value of medium was 8.0, the inoculum was 6.7% (v/v). The batch fermentation kinetic models were established (cell growth kinetic model, substrate depletion kinetic model, production formation kinetic model) bases on Logistic and Luedeking-Piret equations. Cell growth kinetic model was dX dt = 0.431X(1 - X15.522) , substrate depletion kinetic model was- dSdt = 1.11dXdt + 0.0002dPdt + 0.0008X , and production formation kinetic model was dt = 133.1dXdt + 222.87X . The correlation coefficients R2 of the three equations were 0.99021, 0.98908 and 0.98812, respectively, which indicated a good correlation between experimental values and models. Therefore, the equations could be used to describe the process of cell growth, enzyme synthesis and substrate consumption by HDYM-04 during batch fermentation. The establishment of batch fermentation kinetic models (cell growth dP kinetic model, substrate depletion kinetic model, production formation kinetic model) could lay a foundation for theory and provide practical instruction for the application of HDYM-04 in industry.