CAJ | 학술논문

　　为了提高微生物肥料生产菌株——巨大芽胞杆菌1013的发酵产芽孢数量，采用响应面法对其发酵培养基进行优化。首先采用单因子试验筛选出最佳碳源和氮源；然后设计基础培养基，采用Plackett-Burman方法对基础培养基中影响发酵产芽胞数各因素的效应进行评价，筛选出有显著效应的因素；最后通过响应面分析法优化主要因素的最佳浓度。结果表明，最佳碳源为糖蜜，最佳氮源豆饼粉和硝酸铵；基础培养基中对产芽孢数有显著效应的因素为糖蜜和豆饼粉，响应面优化后这2个因素的最佳含量为：糖蜜4.17%，豆饼粉1.53%。采用优化后的培养基进行摇瓶发酵，巨大芽胞杆菌的发酵产芽胞数为(89.34±3.87)×108 cfu/mL，比基础培养基提高了3.35倍。
　　위료제고미생물비료생산균주——거대아포간균1013적발효산아포수량，채용향응면법대기발효배양기진행우화。수선채용단인자시험사선출최가탄원화담원；연후설계기출배양기，채용Plackett-Burman방법대기출배양기중영향발효산아포수각인소적효응진행평개，사선출유현저효응적인소；최후통과향응면분석법우화주요인소적최가농도。결과표명，최가탄원위당밀，최가담원두병분화초산안；기출배양기중대산아포수유현저효응적인소위당밀화두병분，향응면우화후저2개인소적최가함량위：당밀4.17%，두병분1.53%。채용우화후적배양기진행요병발효，거대아포간균적발효산아포수위(89.34±3.87)×108 cfu/mL，비기출배양기제고료3.35배。
The response surface methodology was used to optimize the fermentation medium of Bacillus megaterium 1013. In the single factor experiment, molasses, soybean powder and NH4NO3, were selected to be the optimal carbon source and nitrogen source, respectively. Then, molasses and soybean powder were found to be the important factors by Plackett-Burman design. The optimal concentration of two important factors was determined by response surface methodology. The results showed that, 4.17% molasses, 1.53% soybean powder. The spores yield of Bacillus megaterium 1013 could reach (89.34±3.87) ×108 cfu/mL by using the optimal medium, which was increased by 3.35 fold compared with the original medium.