CAJ | 학술논문

　　本研究致力于克隆油棕低温相应转录因子基因CBF。以NCBI网站上公布CBF基因设计引物，对油棕的基因组进行扩增，并对其进行回收，转化和测序。总共测了11个克隆，测序结果显示这11个克隆中有3个不同拷贝的CBF基因序列，它们序列之间的同源性分别是99%，92%和91%。将克隆的CBF基因与不同物种的CBF基因进行了聚类分析，分析结果显示：这些不同物种的CBF基因可以分为3类，克隆的油棕的3个 CBF 基因被聚在一块，此外，油棕 CBF 基因与 HvCBF1，OsDREB1E，HvCBF11以及TaCBF11的同源性较高。采用同源序列法，克隆了3个拷贝的 CBF 基因，通过 Blast 比对发现，克隆的CBF基因与NCBI数据库现有的CBF基因具有很高的同源性。这些工作将为油棕抗寒的分子选育奠定基础。
　　본연구치력우극륭유종저온상응전록인자기인CBF。이NCBI망참상공포CBF기인설계인물，대유종적기인조진행확증，병대기진행회수，전화화측서。총공측료11개극륭，측서결과현시저11개극륭중유3개불동고패적CBF기인서렬，타문서렬지간적동원성분별시99%，92%화91%。장극륭적CBF기인여불동물충적CBF기인진행료취류분석，분석결과현시：저사불동물충적CBF기인가이분위3류，극륭적유종적3개 CBF 기인피취재일괴，차외，유종 CBF 기인여 HvCBF1，OsDREB1E，HvCBF11이급TaCBF11적동원성교고。채용동원서렬법，극륭료3개고패적 CBF 기인，통과 Blast 비대발현，극륭적CBF기인여NCBI수거고현유적CBF기인구유흔고적동원성。저사공작장위유종항한적분자선육전정기출。
A total of 11 clones were sequenced. Sequencing results showed that there were three copies of CBF genes in the 11 clones, among which similarities were 99%, 92% and 91% respectively. Moreover, the three cloned CBF genes were assigned with CBF genes from other different species. Assignment result showed that the cloned CBF genes of oil palm were clustered together. Moreover, there was higher similarity between the three cloned CBF genes of oil palm and HvCBF1, OsDREB1E, HvCBF11 as well as TaCBF11. Using homologous cloning, three CBF genes were cloned. By blast search, the cloned CBF genes have high similar with available CBF genes in NCBI website. The work will have a help for molecular selection of cold resistant variety in oil palm.