CAJ | 학술논문

　　目的对乙胺吡嗪利福异烟片Ⅱ的溶出度测定方法进行改进。方法色谱柱为Kromasil C18（5μm,4.6mmI.D.×150mm），流动相为磷酸盐缓冲液-乙腈梯度洗脱，检测波长为238nm，流速为1.5mL?min-1，进样量为20μL。结果利福平在0.1279 mg?mL-1～0.1918mg?mL-1，异烟肼在0.06360 mg?mL-1～0.09540 mg?mL-1，吡嗪酰胺在0.3435 mg?mL-1～0.5153mg?mL-1范围内线性关系良好。平均回收率为利福平98.3%，异烟肼100.0%，吡嗪酰胺99.8%。RSD为利福平0.4%，异烟肼0.4%，吡嗪酰胺0.8%。结论该方法专属性强，稳定性好，准确度高，可用于乙胺吡嗪利福异烟片Ⅱ的溶出度检查。
　　목적대을알필진리복이연편Ⅱ적용출도측정방법진행개진。방법색보주위Kromasil C18（5μm,4.6mmI.D.×150mm），류동상위린산염완충액-을정제도세탈，검측파장위238nm，류속위1.5mL?min-1，진양량위20μL。결과리복평재0.1279 mg?mL-1～0.1918mg?mL-1，이연정재0.06360 mg?mL-1～0.09540 mg?mL-1，필진선알재0.3435 mg?mL-1～0.5153mg?mL-1범위내선성관계량호。평균회수솔위리복평98.3%，이연정100.0%，필진선알99.8%。RSD위리복평0.4%，이연정0.4%，필진선알0.8%。결론해방법전속성강，은정성호，준학도고，가용우을알필진리복이연편Ⅱ적용출도검사。
Objective: To improve dissolution of Rifampicin, Isoniazid, Pyrazinamide and Ethambutol hydrochloride Tablets Ⅱ.Methods: Chromatography was performed on the Kromasil C18(5μm,4.6mmI.D.×150mm),using phosphate buffer and acetonitrile to elute gradiently as the mobile phase, the detection wavelength was 238nm, the flow rate was 1.5mL?min-1, the injection volume was 20 μL. Results: The linear ranges were 0.1279~0.1918mg?mL-1 for Rifampicin, 0.06360~0.09540mg?mL-1 for Isoniazid and 0.3435~0.5153mg?mL-1 for Ethambutol hydrochloride, the recovery were 98.3% for Rifampicin, 100.0% for Isoniazid and 99.8% for Ethambutol hydrochloride, RSD were 0.4% for Rifampicin, 0.4% for Isoniazid and 0.8% for Ethambutol hydrochloride. Conclusion: The method is specified, stable, with good accuracy, which can be used for dissolution of Rifampicin, Isoniazid, Pyrazinamide and Ethambutol hydrochloride Tablets Ⅱ.