CAJ | 학술논문

　　研究不同外植体和植物激素对半夏类原球茎诱导的影响，并在此基础上建立半夏高频再生体系。以贵州大方‘圆珠’半夏为材料，进行了半夏类原球茎的诱导、分化和驯化移栽技术的试验研究。块茎诱导类原球茎的适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L，诱导率和增殖倍数分别为86.67%和17.40。叶片和叶柄则采用MS+6-BA 1.0 mg/L+NAA 0.1 mg/L配比的培养基效果较好，类原球茎诱导率均可达50%以上，最大增殖倍数分别为12.80和8.6。炼苗移栽时，选用直径为0.5 cm、根数为9.4个/株、根长为3.71 cm 左右的组培苗，移栽成活率高且植株生长良好。组培苗移栽基质为园土和珍珠岩体积比1：1最好。在适宜的植物激素配比下，可从合适的半夏外植体上诱导出类原球茎，建立半夏高频再生体系，为半夏的种质资源保存、基因工程育种和药用次生代谢产物的生产等奠定基础。
　　연구불동외식체화식물격소대반하류원구경유도적영향，병재차기출상건립반하고빈재생체계。이귀주대방‘원주’반하위재료，진행료반하류원구경적유도、분화화순화이재기술적시험연구。괴경유도류원구경적괄의배양기위MS+6-BA 1.0 mg/L+NAA 0.3 mg/L，유도솔화증식배수분별위86.67%화17.40。협편화협병칙채용MS+6-BA 1.0 mg/L+NAA 0.1 mg/L배비적배양기효과교호，류원구경유도솔균가체50%이상，최대증식배수분별위12.80화8.6。련묘이재시，선용직경위0.5 cm、근수위9.4개/주、근장위3.71 cm 좌우적조배묘，이재성활솔고차식주생장량호。조배묘이재기질위완토화진주암체적비1：1최호。재괄의적식물격소배비하，가종합괄적반하외식체상유도출류원구경，건립반하고빈재생체계，위반하적충질자원보존、기인공정육충화약용차생대사산물적생산등전정기출。
The influence of different explants and phytohormone on the protocorm-like bodies induction from Pinellia ternate was studied to establish a protocol of a rapid propagation of Pinellia ternate.‘Yuanzhu’ Pinellia ternate of Dafang in Guizhou Province were used as explants to conduct protocrom-like bodies induction, differentiation and domestication transplanting technology test research. The proper protocrom-like bodies’induction medium was MS+6-BA 1.0 mg/L+ NAA 0.3 mg/L. Induction rate and the multiplication factor were 86.67% and 17.40. Leaves and petioles formed protocorm-like bodies (above 50%) on the medium of the MS + 6-BA 1.0 mg/L + NAA 0.1 mg/L, and the biggest proliferation multiple was 12.80 and 8.6, respectively. 0.5 cm, 9.4 root number/plant, 3.71 cm root length tissue culture seedlings which has high survival rate and grows well was the best tissue culture seedling for transplanting. The best growth in the matrix was based on the ratio of soil:perlite=1:1(V:V). Protocorm-like bodies of Pinellia ternate could be induced in the suitable ratio of plant hormones from the right explant and high frequency regeneration system of Pinellia ternate could be established, which laid a foundation for Pinellia ternate germplasm resources conservation, gene engineering breeding, medicinal secondary metabolite production and so on.