CAJ | 학술논문

　　为探索蔷薇科植物DELLA蛋白的结构特征和亲缘进化关系，以蔷薇科植物苹果、梨和玫瑰等19个 DELLA 蛋白为试材，利用 expasy、PSORT 和 PROSITE 数据库、TM-HMM 方法、SignalP4.0Server、CDD、DNAMAN和MEGA version5.3等软件对蛋白质进行了生物信息学分析。结果表明：蔷薇科植物中19个DELLA蛋白氨基酸序列组成成分和理化性质差异不明显，均为非跨膜类亲水性蛋白，且不含信号肽；不同DELLA蛋白之间同源性较高，达到74.38%以上，具有DELLA和GRAS 2个保守结构域，功能位点是GRAS；19个DELLA蛋白N端同源性较低，但是存在TVHYNP、VHIID和RVER等DELLA蛋白典型结构域；进化树显示梨属和蔷薇属属内植物DELLA蛋白亲缘关系较近，苹果属内植物亲缘关系相差较远。本研究的开展为蔷薇科植物遗传演化研究提供理论依据。
　　위탐색장미과식물DELLA단백적결구특정화친연진화관계，이장미과식물평과、리화매괴등19개 DELLA 단백위시재，이용 expasy、PSORT 화 PROSITE 수거고、TM-HMM 방법、SignalP4.0Server、CDD、DNAMAN화MEGA version5.3등연건대단백질진행료생물신식학분석。결과표명：장미과식물중19개DELLA단백안기산서렬조성성분화이화성질차이불명현，균위비과막류친수성단백，차불함신호태；불동DELLA단백지간동원성교고，체도74.38%이상，구유DELLA화GRAS 2개보수결구역，공능위점시GRAS；19개DELLA단백N단동원성교저，단시존재TVHYNP、VHIID화RVER등DELLA단백전형결구역；진화수현시리속화장미속속내식물DELLA단백친연관계교근，평과속내식물친연관계상차교원。본연구적개전위장미과식물유전연화연구제공이론의거。
In order to explore the structural feature and phylogenetic analysis of DELLA protein in Rosaceous plants, 19 DELLA proteins of Malus, Pyrus and Rose in Rosaceous plants were analyzed by using expasy, PSORT and PROSITE date bank, TM-HMM, SignalP4.0Server, CDD, DNAMAN and MEGA version 5.3 softwares. The results showed that all DELLA proteins were non-transmembrane hydrophilic proteins and without the signal peptides, and the difference was insignificant among their amino acid composition, physical and chemical characteristics. There was high homology among the different DELLA proteins, reached above 74.38% . Both DELLA and GRAS had two conserved domains, and the function sites were GRAS. The homology of the N-terminal of the 19 DELLA proteins were low, however they had the DELLA protein typical domains such as TVHYNP, VHIID and RVER etc. Phylogenetic tree analysis showed DELLA proteins had the closest relationships in Pyrus and Rose plants, and the distant relationships in Malus plants. The study provided theoretical basis for the genetic evolution of Rosaceous plants.