CAJ | 학술논문

　　N基因起源于烟草野生种粘毛烟草(Nicotiana glutinosa)，属于TIR-NBS-LRR类抗病基因，介导烟草花叶病毒(tobacco mosaic virus, TMV)的抗性，通过转座子标签法得到克隆，目前，N-TMV互作是研究最早且最多的植物-病原菌互作模型之一。笔者从转录产物、表达特征、温度敏感性、对应无毒基因等研究内容回顾了N基因在结构、表达、作用机理等方面的研究进展及现状，归纳了以N基因为TMV抗源在烤烟遗传育种中的应用及取得的成果，并从抗TMV机制研究、抗TMV种质鉴定、种质资源利用等方面对N基因在烤烟抗TMV育种中的高效利用提出了展望。
　　N기인기원우연초야생충점모연초(Nicotiana glutinosa)，속우TIR-NBS-LRR류항병기인，개도연초화협병독(tobacco mosaic virus, TMV)적항성，통과전좌자표첨법득도극륭，목전，N-TMV호작시연구최조차최다적식물-병원균호작모형지일。필자종전록산물、표체특정、온도민감성、대응무독기인등연구내용회고료N기인재결구、표체、작용궤리등방면적연구진전급현상，귀납료이N기인위TMV항원재고연유전육충중적응용급취득적성과，병종항TMV궤제연구、항TMV충질감정、충질자원이용등방면대N기인재고연항TMV육충중적고효이용제출료전망。
The N gene, cloned from wild tobacco (Nicotiana glutinosa), is a type member of the Toll-IL-1 receptor homology region (TIR)-nucleotide binding site (NBS)-leucinerich repeat region (LRR) class of R genes and confers resistance to tobacco mosaic virus (TMV). The N gene was isolated by insertional mutagenesis using the Activator (Ac) transposon of maize, and N-TMV interaction was one of the earliest and most studied models of plant-pathogen interaction. In this article, the research progress of N gene in structure, expression, action mechanism were reviewed from the transcription products, expression characteristics, temperature sensitivity, corresponding avirulence gene and other aspects. The efficient using and achievements of N gene in TMV resistance breeding were summarized. And the prospect of efficient using of N gene was given from three aspects, which were the mechanism research of resistant to TMV, identification and utilization of germplasm resources.