中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
19期
35-38
,共4页
张盼盼%施宠%李桂真%王纯利
張盼盼%施寵%李桂真%王純利
장반반%시총%리계진%왕순리
禾本科牧草%内生真菌%带菌率%镜检
禾本科牧草%內生真菌%帶菌率%鏡檢
화본과목초%내생진균%대균솔%경검
gramineous grass%endophytic fungus%carrying rate%microscopical detection
植物-内生真菌复合体的相互作用对干旱区禾草的生态适应性具有重要的影响。本研究旨在通过检测新疆各种禾草不同部位的内生真菌带菌率及其形态,为内生真菌资源今后的开发利用提供依据和材料。采用苯胺兰染色方法对禾草种子的糊粉层、种皮、植物叶鞘的内生真菌带菌率进行了检测。结果显示,披碱草的叶鞘、糊粉层、种皮均检测到菌丝,并且带菌率很高;绝大部分被调查的禾草叶鞘内未检测到内生菌;很多植物种子糊粉层中检测不到内生菌;然而种皮带菌率很高,大部分都可以检测到菌丝;因而可以看出新疆禾草有很多并且有很高带菌的共生体,为研究寄主及内生菌的生理生化特性提供丰富的资源。
植物-內生真菌複閤體的相互作用對榦旱區禾草的生態適應性具有重要的影響。本研究旨在通過檢測新疆各種禾草不同部位的內生真菌帶菌率及其形態,為內生真菌資源今後的開髮利用提供依據和材料。採用苯胺蘭染色方法對禾草種子的糊粉層、種皮、植物葉鞘的內生真菌帶菌率進行瞭檢測。結果顯示,披堿草的葉鞘、糊粉層、種皮均檢測到菌絲,併且帶菌率很高;絕大部分被調查的禾草葉鞘內未檢測到內生菌;很多植物種子糊粉層中檢測不到內生菌;然而種皮帶菌率很高,大部分都可以檢測到菌絲;因而可以看齣新疆禾草有很多併且有很高帶菌的共生體,為研究寄主及內生菌的生理生化特性提供豐富的資源。
식물-내생진균복합체적상호작용대간한구화초적생태괄응성구유중요적영향。본연구지재통과검측신강각충화초불동부위적내생진균대균솔급기형태,위내생진균자원금후적개발이용제공의거화재료。채용분알란염색방법대화초충자적호분층、충피、식물협초적내생진균대균솔진행료검측。결과현시,피감초적협초、호분층、충피균검측도균사,병차대균솔흔고;절대부분피조사적화초협초내미검측도내생균;흔다식물충자호분층중검측불도내생균;연이충피대균솔흔고,대부분도가이검측도균사;인이가이간출신강화초유흔다병차유흔고대균적공생체,위연구기주급내생균적생리생화특성제공봉부적자원。
The interaction of plant-endophytic association was very significant for the ecological adaptability of the grasses in arid region. This research aimed at the endophytic fungi carrying rate and their morphology through microscopically inspecting the different parts of several gramineous grasses collected in Xinjiang, which could provide information and material for the future application of such endophytic fungal resources. Aniline blue staining method was applied for detection of endophytic fungi carrying rate in the seed skin and aleuronic layer and leaf sheaths. The result showed that leaf sheaths, aleurone layer, seed coat of Elymus was detected in mycelia, and carrier rate was high; the vast majority of respondents were not detected in leaf sheath of endophytic bacteria; many plant seed aleurone layer was not detected in endophytic bacteria; however the belt strain rate was very high, most of them could be detected and hyphae; Xinjiang gramineous grasses had many symbiotic bacteria, and provided rich resources for the physiological and biochemical characteristics of host and endophyte.