中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
20期
1-2
,共2页
刘湘%宁勇%薛津若%陈琳%何立%蒋明森%陈刚
劉湘%寧勇%薛津若%陳琳%何立%蔣明森%陳剛
류상%저용%설진약%진림%하립%장명삼%진강
日本血吸虫%童虫%早期诊断抗原%模拟表位%筛选
日本血吸蟲%童蟲%早期診斷抗原%模擬錶位%篩選
일본혈흡충%동충%조기진단항원%모의표위%사선
Schistosoma japonicum%Schistosomula%Early diagnositic antigen%Minotopes%Screen
目的：利用噬菌体随机12肽库技术筛选日本血吸虫感染早期诊断抗原。方法：用日本血吸虫感染后21 d 兔血清从噬菌体12肽库中经3轮筛选，有效地富集与早期感染兔血清有亲和力的噬菌体克隆。随机挑取11个单克隆分别纯化、扩增，随后采用 ELISA、DOT-ELISA 等检测方法，挑选出与早期感染兔血清有高亲和力的噬菌体克隆。结果：挑选出3个与感染后21 d 兔血清具有高亲和力的噬菌体单克隆。结论：用感染后21 d 兔血清从噬菌体随机12肽库中能筛选到日本血吸虫童虫早期诊断抗原模拟表位。
目的：利用噬菌體隨機12肽庫技術篩選日本血吸蟲感染早期診斷抗原。方法：用日本血吸蟲感染後21 d 兔血清從噬菌體12肽庫中經3輪篩選，有效地富集與早期感染兔血清有親和力的噬菌體剋隆。隨機挑取11箇單剋隆分彆純化、擴增，隨後採用 ELISA、DOT-ELISA 等檢測方法，挑選齣與早期感染兔血清有高親和力的噬菌體剋隆。結果：挑選齣3箇與感染後21 d 兔血清具有高親和力的噬菌體單剋隆。結論：用感染後21 d 兔血清從噬菌體隨機12肽庫中能篩選到日本血吸蟲童蟲早期診斷抗原模擬錶位。
목적：이용서균체수궤12태고기술사선일본혈흡충감염조기진단항원。방법：용일본혈흡충감염후21 d 토혈청종서균체12태고중경3륜사선，유효지부집여조기감염토혈청유친화력적서균체극륭。수궤도취11개단극륭분별순화、확증，수후채용 ELISA、DOT-ELISA 등검측방법，도선출여조기감염토혈청유고친화력적서균체극륭。결과：도선출3개여감염후21 d 토혈청구유고친화력적서균체단극륭。결론：용감염후21 d 토혈청종서균체수궤12태고중능사선도일본혈흡충동충조기진단항원모의표위。
Objective:To screen the simulating antigen epitopes related with early diagnositic antigens of S.j by 12-mers random peptide library. Method:After performing three rounds of panning by using the affinity selection with infected rabbit sera at 21th day,target specific phages were effectively enriched from a random 12-peptides phage library. 11 positive clones selected randomly from them were compared and their antigenic ability was identified by ELISA. By using ELISA,phage clones with high affinity to infected rabbit sera were obtained. Result:3 of the 11 monoclonal phages were obtained. Conclusion:The positive phage simulating epitopes of Schistosomula of Schistosoma japonicum can been obtained from a random 12-peptides phage library.
