中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2013年
16期
42-43,45
,共3页
郭水柱%袁卫斌%张文峰%车彦忠
郭水柱%袁衛斌%張文峰%車彥忠
곽수주%원위빈%장문봉%차언충
滋补生发丸%2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷%HPLC法
滋補生髮汍%2,3,5,4′-四羥基二苯乙烯-2-O-β-D-葡萄糖苷%HPLC法
자보생발환%2,3,5,4′-사간기이분을희-2-O-β-D-포도당감%HPLC법
Zibu Shengfa pills%2,3,5,4′-Tetrahydroxy-stilbene-2-O-β-D-glucoside%HPLC
目的：建立滋补生发丸中2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量的测定方法。方法：采用HPLC法，用ZORBAX SB-C18（250mm×4.6mm，5μm）色谱柱，乙腈-水（20∶80）为流动相，流速1.0ml/min，检测波长为320nm。结果：2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0257～0.4112μg浓度范围内线性关系良好（γ＝0.9992）。平均回收率为98.78%，RSD为0.84%（n＝6）。结论：方法简便，结果准确，可用于滋补生发丸的定量。
目的：建立滋補生髮汍中2，3，5，4′-四羥基二苯乙烯-2-O-β-D-葡萄糖苷的含量的測定方法。方法：採用HPLC法，用ZORBAX SB-C18（250mm×4.6mm，5μm）色譜柱，乙腈-水（20∶80）為流動相，流速1.0ml/min，檢測波長為320nm。結果：2，3，5，4′-四羥基二苯乙烯-2-O-β-D-葡萄糖苷在0.0257～0.4112μg濃度範圍內線性關繫良好（γ＝0.9992）。平均迴收率為98.78%，RSD為0.84%（n＝6）。結論：方法簡便，結果準確，可用于滋補生髮汍的定量。
목적：건립자보생발환중2，3，5，4′-사간기이분을희-2-O-β-D-포도당감적함량적측정방법。방법：채용HPLC법，용ZORBAX SB-C18（250mm×4.6mm，5μm）색보주，을정-수（20∶80）위류동상，류속1.0ml/min，검측파장위320nm。결과：2，3，5，4′-사간기이분을희-2-O-β-D-포도당감재0.0257～0.4112μg농도범위내선성관계량호（γ＝0.9992）。평균회수솔위98.78%，RSD위0.84%（n＝6）。결론：방법간편，결과준학，가용우자보생발환적정량。
Objective:To establish a method for determining 2, 3, 5, 4′-Tetrahydroxy-stilbene-2-O-β-D-glucoside in Zibu Shengfa pills. Methods: The HPLC method was used with ZORBAX SB-C18 column (250×4.6mm, 5μm), and acetonitrile-Water (20:80) was used as mobile phase. Flow rate was 1.0ml/min, detection wavelength was 320nm. Results: 2, 3, 5, 4′-Tetrahydroxy-stilbene-2-O-β-D-glucoside in 0.0257~0.4112μg showed a good linear relationship (γ=0.9992). The average recovery was 98.78%, and RSD was 0.84% (n=6). Conclusion: This method is simple, results are accurate, and can provide a scientic foundation for controlling the quality of Zibu Shengfa pills.
