吉林农业大学学报
吉林農業大學學報
길임농업대학학보
JOURNAL OF JILIN AGRICUL TURAL UNIVERSITY
2014年
2期
139-143
,共5页
姜龙%慈佳宾%崔学宇%张野%南楠%李继竹%杨伟光
薑龍%慈佳賓%崔學宇%張野%南楠%李繼竹%楊偉光
강룡%자가빈%최학우%장야%남남%리계죽%양위광
玉米%单倍体%诱导率%加倍率%生态环境
玉米%單倍體%誘導率%加倍率%生態環境
옥미%단배체%유도솔%가배솔%생태배경
maize%haploid%induction rate%doubling rate%ecological environment
以8个不同基因型组合为母本、玉米单倍体诱导系JS6-1为父本,在三亚、长春和长岭3种生态环境下研究了单倍体的诱导率、自然加倍率和秋水仙素加倍率。结果表明:不同地点和不同基因型材料的玉米单倍体诱导率、自然加倍率和秋水仙素加倍率之间差异显著。在三亚的单倍体诱导率、自然加倍率和秋水仙素加倍率显著高于长春和长岭,长春的自然加倍率和秋水仙素加倍率显著高于长岭,但单倍体诱导率无显著差异,三亚更适合玉米单倍体诱导和加倍。不同基因型组合的诱导率和加倍率也存在显著差异,由DH系L16所配组合J13×L16(先锋父本×先锋父本DH系)的诱导率和加倍率均显著高于其他组合。选择合适生态条件和基础材料,加强田间管理是提高单倍体育种效率的关键措施。
以8箇不同基因型組閤為母本、玉米單倍體誘導繫JS6-1為父本,在三亞、長春和長嶺3種生態環境下研究瞭單倍體的誘導率、自然加倍率和鞦水仙素加倍率。結果錶明:不同地點和不同基因型材料的玉米單倍體誘導率、自然加倍率和鞦水仙素加倍率之間差異顯著。在三亞的單倍體誘導率、自然加倍率和鞦水仙素加倍率顯著高于長春和長嶺,長春的自然加倍率和鞦水仙素加倍率顯著高于長嶺,但單倍體誘導率無顯著差異,三亞更適閤玉米單倍體誘導和加倍。不同基因型組閤的誘導率和加倍率也存在顯著差異,由DH繫L16所配組閤J13×L16(先鋒父本×先鋒父本DH繫)的誘導率和加倍率均顯著高于其他組閤。選擇閤適生態條件和基礎材料,加彊田間管理是提高單倍體育種效率的關鍵措施。
이8개불동기인형조합위모본、옥미단배체유도계JS6-1위부본,재삼아、장춘화장령3충생태배경하연구료단배체적유도솔、자연가배솔화추수선소가배솔。결과표명:불동지점화불동기인형재료적옥미단배체유도솔、자연가배솔화추수선소가배솔지간차이현저。재삼아적단배체유도솔、자연가배솔화추수선소가배솔현저고우장춘화장령,장춘적자연가배솔화추수선소가배솔현저고우장령,단단배체유도솔무현저차이,삼아경괄합옥미단배체유도화가배。불동기인형조합적유도솔화가배솔야존재현저차이,유DH계L16소배조합J13×L16(선봉부본×선봉부본DH계)적유도솔화가배솔균현저고우기타조합。선택합괄생태조건화기출재료,가강전간관리시제고단배체육충효솔적관건조시。
Using inducer JS6 -1 as male parent,8 different genetic combinations as female parent, statistics on maize haploid induction rate and doubling rate in Changchun,Changling and Sanya under 3 different ecological conditions were analyzed .The results indicated that there were significant differences on haploid induction rate,natural and Colchicine doubling rates of maize haploid in different places and on different genotypes .Induction rate,natural and Colchicine doubling rates were significantly higher than those in Changchun and Changling .Natural and Colchicine doubling rates in Changchun were signif-icantly higher than those in Changling,but no significant differences in haploid induction rate .Maize in-duction and double haploid were more suitable for Sanya .There were also significant differences in induc-tion and doubling rates of different genotypes .From DH1 6 line,induction and doubling rates of combina-tions L1 3 ×L1 6 (pioneer male parent ×pioneer male parent DH line)were significantly higher than those of other combinations .Selecting appropriate location and basic materials and strengthening field manage-ment were the key measures to improve the efficiency of maize haploid breeding .