中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2014年
13期
43-44,45
,共3页
卵巢早衰%四二杞乌颗粒%雷公藤多甙片造模%抗苗勒管激素
卵巢早衰%四二杞烏顆粒%雷公籐多甙片造模%抗苗勒管激素
란소조쇠%사이기오과립%뢰공등다대편조모%항묘륵관격소
POF%Sierqiwu particles%TWP modeling%AMH
目的：研究四二杞乌颗粒对雷公藤多甙片（TWP）所致的卵巢早衰（POF）模型大鼠血清抗苗勒管激素（AMH）及性激素的水平变化，探讨抗苗勒管激素（AMH）在早期预测和诊断 POF 中的价值。方法40只12周龄健康雌性 Wistar 大鼠随机分为造模组（30只）空白对照组（10只）。造模组成功建立 TWP 所致的大鼠 POF 模型后，将模型组随机分为四二杞乌颗粒实验组、己烯雌酚对照组、空白对照组（每组10只），再分别给予四二杞乌颗粒混悬液、己烯雌酚混悬液、等体积的生理盐水（模型对照组和空白对照组）连续灌胃14 d。检测大鼠血清抗苗勒管激素、促卵泡激素、黄体生成素、雌二醇水平，观察大鼠卵巢组织形态和动情周期变化。结果雷公藤多甙片所致的卵巢早衰大鼠血清AMH、E2水平较空白对照组显著降低（P ＜0.05），FSH、LH 水平较空白对照组显著升高（P ＜0.05）；四二杞乌颗粒治疗后，可使大鼠血清 AMH、E2、FSH、LH 水平接近空白对照组水平，并改善大鼠卵巢组织形态和动情周期。结论四二杞乌颗粒治疗卵巢早衰大鼠效果好， AMH 可作为卵巢早衰早期预测和诊断指标之一。
目的：研究四二杞烏顆粒對雷公籐多甙片（TWP）所緻的卵巢早衰（POF）模型大鼠血清抗苗勒管激素（AMH）及性激素的水平變化，探討抗苗勒管激素（AMH）在早期預測和診斷 POF 中的價值。方法40隻12週齡健康雌性 Wistar 大鼠隨機分為造模組（30隻）空白對照組（10隻）。造模組成功建立 TWP 所緻的大鼠 POF 模型後，將模型組隨機分為四二杞烏顆粒實驗組、己烯雌酚對照組、空白對照組（每組10隻），再分彆給予四二杞烏顆粒混懸液、己烯雌酚混懸液、等體積的生理鹽水（模型對照組和空白對照組）連續灌胃14 d。檢測大鼠血清抗苗勒管激素、促卵泡激素、黃體生成素、雌二醇水平，觀察大鼠卵巢組織形態和動情週期變化。結果雷公籐多甙片所緻的卵巢早衰大鼠血清AMH、E2水平較空白對照組顯著降低（P ＜0.05），FSH、LH 水平較空白對照組顯著升高（P ＜0.05）；四二杞烏顆粒治療後，可使大鼠血清 AMH、E2、FSH、LH 水平接近空白對照組水平，併改善大鼠卵巢組織形態和動情週期。結論四二杞烏顆粒治療卵巢早衰大鼠效果好， AMH 可作為卵巢早衰早期預測和診斷指標之一。
목적：연구사이기오과립대뢰공등다대편（TWP）소치적란소조쇠（POF）모형대서혈청항묘륵관격소（AMH）급성격소적수평변화，탐토항묘륵관격소（AMH）재조기예측화진단 POF 중적개치。방법40지12주령건강자성 Wistar 대서수궤분위조모조（30지）공백대조조（10지）。조모조성공건립 TWP 소치적대서 POF 모형후，장모형조수궤분위사이기오과립실험조、기희자분대조조、공백대조조（매조10지），재분별급여사이기오과립혼현액、기희자분혼현액、등체적적생리염수（모형대조조화공백대조조）련속관위14 d。검측대서혈청항묘륵관격소、촉란포격소、황체생성소、자이순수평，관찰대서란소조직형태화동정주기변화。결과뢰공등다대편소치적란소조쇠대서혈청AMH、E2수평교공백대조조현저강저（P ＜0.05），FSH、LH 수평교공백대조조현저승고（P ＜0.05）；사이기오과립치료후，가사대서혈청 AMH、E2、FSH、LH 수평접근공백대조조수평，병개선대서란소조직형태화동정주기。결론사이기오과립치료란소조쇠대서효과호， AMH 가작위란소조쇠조기예측화진단지표지일。
Objective To study the changes of serum the anti-Müllerian hormone (AMH) and sex hormones of Sierqiwu particles on premature ovarian failure (POF) rats induced by Tripterygium wilfordii polyglycoside (TWP), and explore the value of the AMH in the early prediction and diagnosis of premature ovarian failure.Methods 40 12-week-old female Wistar rats were randomly divided into model group (n=30) and blank control group (n=10), the first three groups to build modules.After establishing successfully rat model of premature ovarian failure with TWP in model group,then they were randomly divided into Sierqiwu particles experimental group, diethylstilbestrol control group, model control group ,and then they were respectively given Sierqiwu particle suspension, diethylstilbestrol suspension, same dose saline (control group and blank model control groups) for 2 weeks. Serum anti-Müllerian hormone(AMH), follicle-stimulating hormone(FSH), luteinizing hormone(LH), estradiol (E2) rat ovarian tissue morphology and estrous cyclewere obtained. Result The level of serum AMH ,E2 were significantly lower,and FSH, LH significantly increased in premature ovarian failure compared with bank group control; but with the treatment of Sierqiwu particles, serum AMH, E2, FSH, LH levels closed to bank group control (normal levels), and meanwhile improved rat ovarian tissue morphology and estrous cycle.Conclusion Effect of Sierqiwu Particles on the Premature Ovarian in the Rats is actively and AMH can be used as one of the predictors of premature ovarian failure.
