中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
27期
5070-5079
,共10页
张宪彧%汪炜%陈伟%陈建梅%徐皓
張憲彧%汪煒%陳偉%陳建梅%徐皓
장헌욱%왕위%진위%진건매%서호
干细胞%骨髓干细胞%绿色荧光蛋白%骨髓间充质干细胞%嵌合体%骨折%趋化因子%基质细胞衍生因子1%集落刺激因子%肝细胞生长因子%单核细胞趋化蛋白1%间质金属蛋白酶9%省级基金%干细胞图片文章
榦細胞%骨髓榦細胞%綠色熒光蛋白%骨髓間充質榦細胞%嵌閤體%骨摺%趨化因子%基質細胞衍生因子1%集落刺激因子%肝細胞生長因子%單覈細胞趨化蛋白1%間質金屬蛋白酶9%省級基金%榦細胞圖片文章
간세포%골수간세포%록색형광단백%골수간충질간세포%감합체%골절%추화인자%기질세포연생인자1%집락자격인자%간세포생장인자%단핵세포추화단백1%간질금속단백매9%성급기금%간세포도편문장
背景:研究表明,骨髓间充质干细胞定向迁移依赖于损伤局部表达趋化因子与细胞表面相应受体的相互作用。然而,哪些趋化因子在介导骨髓间充质干细胞向骨折部位定向迁移过程中起关键作用,目前尚不清楚。目的:对内源性骨髓间充质干细胞进行示踪,观察其在骨修复中的作用;检测并筛选出骨折微环境中表达量高且与骨髓间充质干细胞趋化规律相关的因子。方法:建立 C57BL/6小鼠荧光/普通骨髓嵌合体,利用嵌合体动物模型制造胫骨骨折模型,在不同时相点检测骨折部位组织绿色荧光蛋白阳性细胞占所有细胞的比例、来源于骨髓间充质干细胞的成骨细胞占全部成骨细胞的比例,判断来源于骨髓的骨髓间充质干细胞在骨折修复中的作用;利用免疫组化等方法检测骨折部位不同时相点趋化因子的蛋白表达水平。结果与结论:骨折局部绿色荧光蛋白阳性细胞占所有细胞比例在术后1,5,14 d 分别为(3.011±0.911)%,(9.031±0.145)%,(12.064±0.145)%;来源于骨髓间充质干细胞的成骨细胞占全部成骨细胞的50%以上;骨折后各时相点微环境中基质细胞衍生因子1、集落刺激因子、肝细胞生长因子、单核细胞趋化蛋白1、间质金属蛋白酶9有不同程度的表达,粒细胞集落刺激因子无明显表达。基质细胞衍生因子1的表达量相对最高。经相关分析认为:基质细胞衍生因子1在骨折微环境中的表达与骨髓间充质干细胞趋化规律具有相关关系。结果表明骨髓内的骨髓间充质干细胞参与骨折修复并在其中起到了重要作用;基质细胞衍生因子1促进骨髓间充质干细胞的定向趋化并参与骨组织的修复。
揹景:研究錶明,骨髓間充質榦細胞定嚮遷移依賴于損傷跼部錶達趨化因子與細胞錶麵相應受體的相互作用。然而,哪些趨化因子在介導骨髓間充質榦細胞嚮骨摺部位定嚮遷移過程中起關鍵作用,目前尚不清楚。目的:對內源性骨髓間充質榦細胞進行示蹤,觀察其在骨脩複中的作用;檢測併篩選齣骨摺微環境中錶達量高且與骨髓間充質榦細胞趨化規律相關的因子。方法:建立 C57BL/6小鼠熒光/普通骨髓嵌閤體,利用嵌閤體動物模型製造脛骨骨摺模型,在不同時相點檢測骨摺部位組織綠色熒光蛋白暘性細胞佔所有細胞的比例、來源于骨髓間充質榦細胞的成骨細胞佔全部成骨細胞的比例,判斷來源于骨髓的骨髓間充質榦細胞在骨摺脩複中的作用;利用免疫組化等方法檢測骨摺部位不同時相點趨化因子的蛋白錶達水平。結果與結論:骨摺跼部綠色熒光蛋白暘性細胞佔所有細胞比例在術後1,5,14 d 分彆為(3.011±0.911)%,(9.031±0.145)%,(12.064±0.145)%;來源于骨髓間充質榦細胞的成骨細胞佔全部成骨細胞的50%以上;骨摺後各時相點微環境中基質細胞衍生因子1、集落刺激因子、肝細胞生長因子、單覈細胞趨化蛋白1、間質金屬蛋白酶9有不同程度的錶達,粒細胞集落刺激因子無明顯錶達。基質細胞衍生因子1的錶達量相對最高。經相關分析認為:基質細胞衍生因子1在骨摺微環境中的錶達與骨髓間充質榦細胞趨化規律具有相關關繫。結果錶明骨髓內的骨髓間充質榦細胞參與骨摺脩複併在其中起到瞭重要作用;基質細胞衍生因子1促進骨髓間充質榦細胞的定嚮趨化併參與骨組織的脩複。
배경:연구표명,골수간충질간세포정향천이의뢰우손상국부표체추화인자여세포표면상응수체적상호작용。연이,나사추화인자재개도골수간충질간세포향골절부위정향천이과정중기관건작용,목전상불청초。목적:대내원성골수간충질간세포진행시종,관찰기재골수복중적작용;검측병사선출골절미배경중표체량고차여골수간충질간세포추화규률상관적인자。방법:건립 C57BL/6소서형광/보통골수감합체,이용감합체동물모형제조경골골절모형,재불동시상점검측골절부위조직록색형광단백양성세포점소유세포적비례、래원우골수간충질간세포적성골세포점전부성골세포적비례,판단래원우골수적골수간충질간세포재골절수복중적작용;이용면역조화등방법검측골절부위불동시상점추화인자적단백표체수평。결과여결론:골절국부록색형광단백양성세포점소유세포비례재술후1,5,14 d 분별위(3.011±0.911)%,(9.031±0.145)%,(12.064±0.145)%;래원우골수간충질간세포적성골세포점전부성골세포적50%이상;골절후각시상점미배경중기질세포연생인자1、집락자격인자、간세포생장인자、단핵세포추화단백1、간질금속단백매9유불동정도적표체,립세포집락자격인자무명현표체。기질세포연생인자1적표체량상대최고。경상관분석인위:기질세포연생인자1재골절미배경중적표체여골수간충질간세포추화규률구유상관관계。결과표명골수내적골수간충질간세포삼여골절수복병재기중기도료중요작용;기질세포연생인자1촉진골수간충질간세포적정향추화병삼여골조직적수복。
BACKGROUND: The oriented migration of bone marrow mesenchymal stem cells may depend on the interaction between local chemotactic factors and cellsurface receptors. However, which chemotactic factors may mediate the oriented migration of bone marrow mesenchymal stem cells towards the fracture site remains unclear. OBJECTIVE: To tag autologous bone marrow mesenchymal stem cells, evaluate its role in bone healing, and detect the highly expressed factors associated with migration of bone marrow mesenchymal stem cells in the microenvironment. METHODS: The fluorescence/chimeric C57BL/6 mouse models were established, then left shankbone fracture models were also produced. The percentages of green fluorescent protein positive cells to al cells at the fracture site and the percentage of osteoblasts differentiated from bone marrow mesenchymal stem cells to al the osteoblasts were detected at different time points. The role of bone marrow mesenchymal stem cells in the fracture repairing was evaluated. The levels of chemotactic factors protein expression at the fracture site in different time points were detected with immunohistochemistry technology. RESULTS AND CONCLUSION: The percentage of green fluorescent protein positive cells to al cells at the fracture site was (3.011±0.911)%, (9.031±0.145)%, (12.064±0.145)% at 1, 5, 14 days postoperatively; and osteoblasts differentiated from bone marrow mesenchymal stem cells accounted for 50% of al the osteoblasts. After fracture, the stromal cel derived factor-1, colony stimulating factor, hepatocyte growth factor, monocyte chemoattractant protein-1, and matrix metal oproteinases-9 were expressed to varying degrees in the microenvironment, while the expression of granulocyte colony-stimulating factor was negative. The expression of stromal cel derived factor-1 in the fracture microenvironment was the highest, mainly due to the migration of bone marrow mesenchymal stem cells. Experimental findings indicate that, autologous bone marrow mesenchymal stem cells participate in and play an important role in bone healing. The stromal cel derived factor-1 plays an important role in promoting bone marrow mesenchymal stem cells migration and promoting bone healing.