CAJ | 학술논문

　　背景：黄芪对神经功能缺损疾病治疗及神经再生的作用已受到神经科学和脑科学研究者的密切关注，其对神经干细胞的影响也成为一个新的探索方向。目的：探索黄芪注射液对大鼠神经干细胞生物活性的影响。方法：分离、培养 Wistar 大鼠胚胎神经干细胞。采用荧光免疫细胞化学法鉴定巢蛋白染色阳性，原代培养细胞传至第2代纯化后，随机分为对照组、50，200，400 g/L 黄芪注射液组分别培养6，12，24 h 后。采用MTT 法检测细胞活性，通过比较细胞活性，选50 g/L 黄芪注射液组诱导分化7 d 后用免疫组化法检测神经元特异性烯醇化酶和胶质纤维酸性蛋白的表达。结果与结论：MTT 显示药物作用6 h，50，200，400 g/L 黄芪注射液组细胞的活性与对照组相比明显升高(P <0.05)；但24 h 后不同质量浓度的黄芪注射液对细胞活性逐渐趋于一致(P >0.05)。免疫组织化学检测显示，与对照组相比，50 g/L 的黄芪注射液组诱导的细胞分化快速，细胞中神经元特异性烯醇化酶阳性细胞数量也明显增加(P <0.05)。实验提示黄芪注射液可以促进神经干细胞增殖，对细胞分化也具有一定促进作用。
　　배경：황기대신경공능결손질병치료급신경재생적작용이수도신경과학화뇌과학연구자적밀절관주，기대신경간세포적영향야성위일개신적탐색방향。목적：탐색황기주사액대대서신경간세포생물활성적영향。방법：분리、배양 Wistar 대서배태신경간세포。채용형광면역세포화학법감정소단백염색양성，원대배양세포전지제2대순화후，수궤분위대조조、50，200，400 g/L 황기주사액조분별배양6，12，24 h 후。채용MTT 법검측세포활성，통과비교세포활성，선50 g/L 황기주사액조유도분화7 d 후용면역조화법검측신경원특이성희순화매화효질섬유산성단백적표체。결과여결론：MTT 현시약물작용6 h，50，200，400 g/L 황기주사액조세포적활성여대조조상비명현승고(P <0.05)；단24 h 후불동질량농도적황기주사액대세포활성축점추우일치(P >0.05)。면역조직화학검측현시，여대조조상비，50 g/L 적황기주사액조유도적세포분화쾌속，세포중신경원특이성희순화매양성세포수량야명현증가(P <0.05)。실험제시황기주사액가이촉진신경간세포증식，대세포분화야구유일정촉진작용。
BACKGROUND: Neuroscience and brain science researches have paid attention to the effect of astragalus membranaceus in the treatment of neurologic impairment disease and neural regeneration. Studying astragalus membranaceus effects on neural stem cells are becoming a new research direction. OBJECTIVE: To explore the effects of astragalus injection on biological viability of rat neural stem cells. METHODS: Neural stem cells of Wistar rats were separated and cultured. Immunofluorescence staining was applied to identify the neural stem cells. The purified cells were gained by the second subcultivation in vitro, and then the cells were randomly divided into control group and astragalus injection groups with various concentrations (50, 200, 400 g/L) to culture for 6, 12 and 24 hours. The activity of cells was tested by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, and then the immunohistochemistry was applied to detect the expressions of neuron-specific enolase and glial fibril ary acidic protein in the 50 g/L astragalus injection group after induced for 7 days. RESULTS AND CONCLUSION: The viability of neural stem cells increased significantly after intervention with different concentrations of astragalus injection for 6 hours as compared with the control group (P < 0.05). However, there was no difference in the cel viability after treated with different concentrations of astragalus injection for 24 hours (P > 0.05). Compared with the control group, the cells in the 50 g/L astragalus group differentiated rapidly, and the number of positive cells for neuron-specific enolase was increased significantly (P < 0.05). The neural stem cells proliferation was hastened, and its differentiation was promoted by the interference of astragalus injection.