中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
27期
5026-5032
,共7页
干细胞%干细胞移植%骨髓间充质干细胞%心肌梗死%细胞移植%糖尿病%心肌%病理变化%细胞增殖%细胞形态%干细胞图片文章
榦細胞%榦細胞移植%骨髓間充質榦細胞%心肌梗死%細胞移植%糖尿病%心肌%病理變化%細胞增殖%細胞形態%榦細胞圖片文章
간세포%간세포이식%골수간충질간세포%심기경사%세포이식%당뇨병%심기%병리변화%세포증식%세포형태%간세포도편문장
stem cells%stem cel transplantation%bone marrow-derived stem cells%myocardial infarction%cel transplantation%diabetes%myocardium%pathological changes%cel proliferation%cel morphology%stem cel photographs-containing paper
背景：目前国内外在干细胞移植治疗心肌梗死的研究中，大多采用正常或者年轻的骨髓间充质干细胞作为移植细胞来源。目的：比较糖尿病和正常大鼠骨髓间充质干细胞移植治疗心肌梗死的疗效差别。方法：无菌条件下获取正常大鼠及糖尿病大鼠的骨髓间充质干细胞，建立大鼠心肌梗死模型并随机分为3组，分别于心肌梗死病灶区注射100μL 含有105-106个 F2代正常大鼠或糖尿病大鼠来源骨髓间充质干细胞的细胞混悬液，空白对照组注射100μL 含体积分数20%胎牛血清的 DMEM。移植后1个月行苏木精-伊红染色检测各组梗死区域心肌组织形态的变化；通过免疫组化方法检测 Bcl-2的表达。结果与结论：通过对细胞形态观察及流式细胞术鉴定，大鼠股骨骨髓贴壁培养可获取纯度较高的骨髓间充质干细胞，绘制细胞生长曲线结果显示正常大鼠骨髓间充质干细胞较糖尿病大鼠骨髓间充质干细胞生长快。移植后1个月，正常大鼠干细胞移植组梗死区域心肌组织形态较糖尿病大鼠干细胞移植组及空白对照组明显改善，梗死区域心肌组织 Bcl-2的表达也高于其他2组。证实正常大鼠来源骨髓间充质干细胞较糖尿病大鼠骨髓间充质干细胞生长明显加快，且糖尿病使骨髓干细胞移植治疗心肌梗死的疗效降低。
揹景：目前國內外在榦細胞移植治療心肌梗死的研究中，大多採用正常或者年輕的骨髓間充質榦細胞作為移植細胞來源。目的：比較糖尿病和正常大鼠骨髓間充質榦細胞移植治療心肌梗死的療效差彆。方法：無菌條件下穫取正常大鼠及糖尿病大鼠的骨髓間充質榦細胞，建立大鼠心肌梗死模型併隨機分為3組，分彆于心肌梗死病竈區註射100μL 含有105-106箇 F2代正常大鼠或糖尿病大鼠來源骨髓間充質榦細胞的細胞混懸液，空白對照組註射100μL 含體積分數20%胎牛血清的 DMEM。移植後1箇月行囌木精-伊紅染色檢測各組梗死區域心肌組織形態的變化；通過免疫組化方法檢測 Bcl-2的錶達。結果與結論：通過對細胞形態觀察及流式細胞術鑒定，大鼠股骨骨髓貼壁培養可穫取純度較高的骨髓間充質榦細胞，繪製細胞生長麯線結果顯示正常大鼠骨髓間充質榦細胞較糖尿病大鼠骨髓間充質榦細胞生長快。移植後1箇月，正常大鼠榦細胞移植組梗死區域心肌組織形態較糖尿病大鼠榦細胞移植組及空白對照組明顯改善，梗死區域心肌組織 Bcl-2的錶達也高于其他2組。證實正常大鼠來源骨髓間充質榦細胞較糖尿病大鼠骨髓間充質榦細胞生長明顯加快，且糖尿病使骨髓榦細胞移植治療心肌梗死的療效降低。
배경：목전국내외재간세포이식치료심기경사적연구중，대다채용정상혹자년경적골수간충질간세포작위이식세포래원。목적：비교당뇨병화정상대서골수간충질간세포이식치료심기경사적료효차별。방법：무균조건하획취정상대서급당뇨병대서적골수간충질간세포，건립대서심기경사모형병수궤분위3조，분별우심기경사병조구주사100μL 함유105-106개 F2대정상대서혹당뇨병대서래원골수간충질간세포적세포혼현액，공백대조조주사100μL 함체적분수20%태우혈청적 DMEM。이식후1개월행소목정-이홍염색검측각조경사구역심기조직형태적변화；통과면역조화방법검측 Bcl-2적표체。결과여결론：통과대세포형태관찰급류식세포술감정，대서고골골수첩벽배양가획취순도교고적골수간충질간세포，회제세포생장곡선결과현시정상대서골수간충질간세포교당뇨병대서골수간충질간세포생장쾌。이식후1개월，정상대서간세포이식조경사구역심기조직형태교당뇨병대서간세포이식조급공백대조조명현개선，경사구역심기조직 Bcl-2적표체야고우기타2조。증실정상대서래원골수간충질간세포교당뇨병대서골수간충질간세포생장명현가쾌，차당뇨병사골수간세포이식치료심기경사적료효강저。
BACKGROUND: Current studies have shown that bone marrow mesenchymal stem cells from normal or young people usual y serve as a source of transplanted cells in stem cel transplantation treatment of myocardial infarction. OBJECTIVE: To compare the therapeutic effects of bone marrow mesenchymal stem cells from diabetic and normal rats on myocardial infarction. METHODS: Under sterile conditions, bone marrow mesenchymal stem cells from normal and diabetic rats were harvested. Then, rat models of myocardial infarction were established and randomly divided into three groups:100 μL cellsuspension containing 105-106 bone marrow mesenchymal stem cells from F2 normal or diabetic rats was injected into myocardial infarction lesions, and 100 μL Dulbecco’s modified Eagle’s medium containing 20%fetal bovine serum was injected serving as blank control. After 1 month, hematoxylin-eosin staining for myocardial infarction lesions was performed for histomorphological observation. Bcl-2 expression was detected by immunohistochemistry method. RESULTS AND CONCLUSION: Based on cel morphology observation and flow cytometry identification, high-purity bone marrow mesenchymal stem cells could be obtained using rat femoral bone marrow adherent culture. Cel growth curve showed that normal rat bone marrow mesenchymal stem cells grew faster than those from diabetic rats. At 1 month after transplantation, histomorphological improvement was seen in the infarcted area after transplantation of normal rat bone marrow mesenchymal stem cells as compared with the other two groups. In addition, the Bcl-2 expression in the infarcted area was higher in the normal rat cel group than the the other two groups. These findings indicate that bone marrow mesenchymal stem cells from normal rats grow faster than those from diabetic rats, and the cells from normal rats have better therapeutic effects on myocardial infarction.
