中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
27期
5005-5011
,共7页
干细胞%干细胞移植%肝硬化%脐血间充质干细胞%移植%定植%归巢%分化%血清白蛋白%纤维化%肝功能%干细胞图片文章
榦細胞%榦細胞移植%肝硬化%臍血間充質榦細胞%移植%定植%歸巢%分化%血清白蛋白%纖維化%肝功能%榦細胞圖片文章
간세포%간세포이식%간경화%제혈간충질간세포%이식%정식%귀소%분화%혈청백단백%섬유화%간공능%간세포도편문장
stem cells%stem cel transplantation%liver cirrhosis%umbilical cord blood mesenchymal stem cells%transplantation%colonization%homing%differentiation%serum albumin%fibrosis%liver function%stem cel
背景：人脐血间充质干细胞移植治疗肝硬化的可行性及机制有待深入探讨。目的：观察经门静脉移植人脐血间充质干细胞对肝硬化大鼠肝功能及组织病理学改变的影响。方法：采用四氯化碳法制备肝硬化大鼠模型，造模成功后，细胞移植组经门静脉注射1 mL BrdU 标记的人脐血间充质干细胞(5×106个)，模型组注射等体积的 PBS；以经门静脉移植1 mL 人脐血间充质干细胞的正常大鼠作为对照。细胞移植后4周，取大鼠尾静脉血及肝脏组织进行检测。结果与结论：细胞移植后4周，与模型组比较，细胞移植组大鼠血清谷丙转氨酶、谷草转氨酶、总胆红素明显降低，而白蛋白明显升高(P <0.01)；肝细胞炎性坏死、脂肪变及肝纤维化程度明显改善(P <0.05或 P <0.01)。免疫组化及免疫荧光染色显示细胞移植组和对照组大鼠肝组织中均有人脐血间充质干细胞的定植，但细胞移植组 BrdU 阳性细胞数目明显多于对照组。RT-PCR 检测结果显示，细胞移植组大鼠肝组织表达人源性细胞角蛋白18和白蛋白 mRNA，而模型组未见。可见人脐血间充质干细胞移植可在一定程度上改善肝硬化大鼠的肝功能及病理损伤，其机制可能与移植细胞在肝硬化大鼠肝内归巢定植并向肝样细胞分化有关。
揹景：人臍血間充質榦細胞移植治療肝硬化的可行性及機製有待深入探討。目的：觀察經門靜脈移植人臍血間充質榦細胞對肝硬化大鼠肝功能及組織病理學改變的影響。方法：採用四氯化碳法製備肝硬化大鼠模型，造模成功後，細胞移植組經門靜脈註射1 mL BrdU 標記的人臍血間充質榦細胞(5×106箇)，模型組註射等體積的 PBS；以經門靜脈移植1 mL 人臍血間充質榦細胞的正常大鼠作為對照。細胞移植後4週，取大鼠尾靜脈血及肝髒組織進行檢測。結果與結論：細胞移植後4週，與模型組比較，細胞移植組大鼠血清穀丙轉氨酶、穀草轉氨酶、總膽紅素明顯降低，而白蛋白明顯升高(P <0.01)；肝細胞炎性壞死、脂肪變及肝纖維化程度明顯改善(P <0.05或 P <0.01)。免疫組化及免疫熒光染色顯示細胞移植組和對照組大鼠肝組織中均有人臍血間充質榦細胞的定植，但細胞移植組 BrdU 暘性細胞數目明顯多于對照組。RT-PCR 檢測結果顯示，細胞移植組大鼠肝組織錶達人源性細胞角蛋白18和白蛋白 mRNA，而模型組未見。可見人臍血間充質榦細胞移植可在一定程度上改善肝硬化大鼠的肝功能及病理損傷，其機製可能與移植細胞在肝硬化大鼠肝內歸巢定植併嚮肝樣細胞分化有關。
배경：인제혈간충질간세포이식치료간경화적가행성급궤제유대심입탐토。목적：관찰경문정맥이식인제혈간충질간세포대간경화대서간공능급조직병이학개변적영향。방법：채용사록화탄법제비간경화대서모형，조모성공후，세포이식조경문정맥주사1 mL BrdU 표기적인제혈간충질간세포(5×106개)，모형조주사등체적적 PBS；이경문정맥이식1 mL 인제혈간충질간세포적정상대서작위대조。세포이식후4주，취대서미정맥혈급간장조직진행검측。결과여결론：세포이식후4주，여모형조비교，세포이식조대서혈청곡병전안매、곡초전안매、총담홍소명현강저，이백단백명현승고(P <0.01)；간세포염성배사、지방변급간섬유화정도명현개선(P <0.05혹 P <0.01)。면역조화급면역형광염색현시세포이식조화대조조대서간조직중균유인제혈간충질간세포적정식，단세포이식조 BrdU 양성세포수목명현다우대조조。RT-PCR 검측결과현시，세포이식조대서간조직표체인원성세포각단백18화백단백 mRNA，이모형조미견。가견인제혈간충질간세포이식가재일정정도상개선간경화대서적간공능급병리손상，기궤제가능여이식세포재간경화대서간내귀소정식병향간양세포분화유관。
BACKGROUND: The feasibility and the mechanism of human umbilical cord blood mesenchymal stem cel transplantation for the treatment of liver cirrhosis need to be discussed in-depth. OBJECTIVE: To observe the effect of human umbilical cord blood mesenchymal stem cel transplantation through portal vein on the liver function and tissue pathological changes of the rats with liver cirrhosis. METHODS: Carbon tetrachloride was used to prepare rat model of liver cirrhosis. After the success of modeling, the rats in the cel transplantation group received portal vein injection of 1 mL 5-bromo-2-deoxyuridine -labeled human umbilical cord blood mesenchymal stem cells (5×106), the model group was injected with the same volume of PBS; the normal rats received 1 mL human umbilical cord blood mesenchymal stem cel transplantation via the portal vein were as the control group. At 4 weeks after transplantation, the rat tail vein blood and liver tissue were obtained for testing. RESULTS AND CONCLUSION: At 4 weeks after cel transplantation, compared with the model group, levels of serum alanine aminotransferase, aspartate aminotransferase and total bilirubin in the cel transplantation group were significantly decreased, while the albumin level was increased significantly (P < 0.01); the liver cel inflammatory necrosis, steatosis and liver fibrosis were improved significantly (P < 0.05 or P < 0.01). Immunohistochemistry and immunofluorescence staining showed that human umbilical cord blood mesenchymal stem cel colonization could be seen in the rat liver tissues of the cel transplantation group and control group, but the number of 5-bromo-2-deoxyuridine-positive cells in cel transplantation group was significantly larger than that in the control group. Reverse transcription-PCR test result showed that the expressions of cytokeratin 18 and albumin mRNA could be observed in the rat liver tissue of the cel transplantation group, but no expression could be seen in the control group. It is visible that human umbilical cord mesenchymal stem cells can improve liver function and pathological damage of liver cirrhosis rats in a certain extent, which may relate with the intrahepatic homing colonization and hepatocyte-like cel differentiation of the transplanted cells in the liver cirrhosis rats.
