CAJ | 학술논문

　　背景：雌激素对脂肪干细胞向脂肪细胞诱导分化会有负向调节作用，但对长期深低温保存的肾脂肪囊来源脂肪间充质干细胞向脂肪细胞分化的效果尚未见报道。目的：探讨雌激素对长期深低温保存的及新鲜提取的肾脂肪囊来源脂肪间充质干细胞成脂分化能力的影响。方法：将长期深低温保存后复苏的及新鲜提取的第3代肾脂肪囊来源脂肪间充质干细胞分为4组进行成脂诱导分化，新鲜+雌激素组和冻存+雌激素组诱导时添加10-7 mol/L 雌激素，新鲜组和冻存组不添加。成脂诱导分化14 d 进行油红 O 染色及成脂量定量检测。结果与结论：长期深低温保存后复苏的第3代肾脂肪囊来源脂肪间充质干细胞与新鲜提取的细胞形态和排列无差异；长期深低温保存后复苏的与新鲜提取的第3代肾脂肪囊来源脂肪间充质干细胞表面抗原分子 CD29、CD44均呈阳性，CD31均呈阴性表达。成脂诱导后可观察到细胞内有脂滴形成，油红 O 染色呈阳性。在成脂诱导分化14 d 对各组进行成脂量比较，新鲜组与新鲜+雌激素组，冻存组与冻存+雌激素组的吸光度之间比较，差异有显著性意义，但新鲜组与冻存组之间差异无显著性意义。结果表明：小剂量雌激素可抑制长期深度低温保存后复苏的肾脂肪囊来源脂肪间充质干细胞的成脂分化；长期深低温保存后复苏的与新鲜提取的第3代肾脂肪囊来源脂肪间充质干细胞在成脂诱导方面无显著性差异。
　　배경：자격소대지방간세포향지방세포유도분화회유부향조절작용，단대장기심저온보존적신지방낭래원지방간충질간세포향지방세포분화적효과상미견보도。목적：탐토자격소대장기심저온보존적급신선제취적신지방낭래원지방간충질간세포성지분화능력적영향。방법：장장기심저온보존후복소적급신선제취적제3대신지방낭래원지방간충질간세포분위4조진행성지유도분화，신선+자격소조화동존+자격소조유도시첨가10-7 mol/L 자격소，신선조화동존조불첨가。성지유도분화14 d 진행유홍 O 염색급성지량정량검측。결과여결론：장기심저온보존후복소적제3대신지방낭래원지방간충질간세포여신선제취적세포형태화배렬무차이；장기심저온보존후복소적여신선제취적제3대신지방낭래원지방간충질간세포표면항원분자 CD29、CD44균정양성，CD31균정음성표체。성지유도후가관찰도세포내유지적형성，유홍 O 염색정양성。재성지유도분화14 d 대각조진행성지량비교，신선조여신선+자격소조，동존조여동존+자격소조적흡광도지간비교，차이유현저성의의，단신선조여동존조지간차이무현저성의의。결과표명：소제량자격소가억제장기심도저온보존후복소적신지방낭래원지방간충질간세포적성지분화；장기심저온보존후복소적여신선제취적제3대신지방낭래원지방간충질간세포재성지유도방면무현저성차이。
BACKGROUND: Estrogen exerts a negative regulatory role in adipogenic differentiation of adipose stem cells, but there is no report concerning estrogen effects on adipogenic differentiation of adipose stem cells from the adipose capsule of kidney after long-term cryopreservation. OBJECTIVE: To explore the impact of estrogen on adipogenic differentiation of fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule or those after long-term cryopreservation. METHODS: Passage 3 long-term cryopreserved and fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule were divided into four groups, al of which were induced to adipogenic cells by induced fluid: fresh cells + 10-7 mol/L estrogen, cryopreserved cells + 10-7 mol/L estrogen, fresh cells and cryopreserved cells groups. Oil red O staining and adipogenic quantitative detection were performed at 14 days after induced adipogenic differentiation. RESULTS AND CONCLUSION: There were no differences in the morphology and arrangement between the cryopreserved and fresh cells. Both cryopreserved and fresh cells expressed CD29 and CD 44, but did not express CD31. Intracel ular lipid droplets were observed after adipogenic differentiation by oil red O staining, and the cells were positive for oil red O staining. The adipogenic volume comparison among the four groups was detected on day 14 after adipogenic differentiation, and the absorbance values showed significant difference between the fresh cells and fresh cells + estrogen groups, as wel as between the cryopreserved cells and cryopreserved cells + estrogen groups, but no difference between the fresh and cryopreserved cells groups. It is proved that low-dose estrogen can inhibit the adipogenic differentiation of long-term cryopreserved adipose-derived mesenchymal stem cells from the kidney adipose capsule; however, there is no significant difference between passage 3 long-term cryopreserved and fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule in adipogenic differentiation.