CAJ | 학술논문

　　背景：有研究表明骨髓间充质干细胞及异体骨可促进骨缺损的修复，但骨髓间充质干细胞复合异体骨对于松质骨缺损的修复效果至今少有报道。目的：观察骨髓间充质干细胞复合异体骨修复兔松质骨缺损效果。方法：在新西兰大白兔双侧股骨外侧髁造成0.6 cm×1.2 cm 的松质骨缺损，一侧设为模型组，骨缺损处植入复合骨髓间充质干细胞的异体骨，另一侧设为对照组，单纯植入异体骨。结果与结论：植入后4，8，12周，大体观察、X 射线检查和苏木精-伊红染色观察结果显示，模型组在新骨成长方面，缺损区修复方面均优于对照组。植入后12周，模型组骨缺损区可见大量骨小梁形成及成熟的板层骨组织，骨缺损基本修复。对照组骨缺损区仅可见大量编织骨形成，骨缺损尚未得到有效修复。模型组Lane-Sandhu 法 X 射线结合组织学观察评分高于对照组(P <0.05)。生物力学检测结果显示，植入后12周，模型组股骨髁最大压力载荷、载荷/应变比值均高于对照组(P <0.05)，最大应变位移较对照组低(P <0.05)。结果证实，骨髓间充质干细胞复合异体骨可有效修复兔股骨髁松质骨缺损，且修复效果明显优于单纯异体骨移植。
　　배경：유연구표명골수간충질간세포급이체골가촉진골결손적수복，단골수간충질간세포복합이체골대우송질골결손적수복효과지금소유보도。목적：관찰골수간충질간세포복합이체골수복토송질골결손효과。방법：재신서란대백토쌍측고골외측과조성0.6 cm×1.2 cm 적송질골결손，일측설위모형조，골결손처식입복합골수간충질간세포적이체골，령일측설위대조조，단순식입이체골。결과여결론：식입후4，8，12주，대체관찰、X 사선검사화소목정-이홍염색관찰결과현시，모형조재신골성장방면，결손구수복방면균우우대조조。식입후12주，모형조골결손구가견대량골소량형성급성숙적판층골조직，골결손기본수복。대조조골결손구부가견대량편직골형성，골결손상미득도유효수복。모형조Lane-Sandhu 법 X 사선결합조직학관찰평분고우대조조(P <0.05)。생물역학검측결과현시，식입후12주，모형조고골과최대압력재하、재하/응변비치균고우대조조(P <0.05)，최대응변위이교대조조저(P <0.05)。결과증실，골수간충질간세포복합이체골가유효수복토고골과송질골결손，차수복효과명현우우단순이체골이식。
BACKGROUND: Some studies have shown that bone marrow mesenchymal stem cells and al ograft bone have a certain role for repairing bone defects, but the effectiveness on cancel ous bone defects is seldom reported so far. OBJECTIVE: To observe the effectiveness of bone marrow mesenchymal stem cells combined with al ogeneic bone on cancel ous bone defects. METHODS: The models of cancel ous bone defects (0.6 cm×1.2 cm) were made artificial y in both condylus lateralis femoris of New Zealand white rabbits: one side served as model group implanted with combination of bone marrow mesenchymal stem cells and al ogeneic bone, and the other side was considered as control group implanted with al ogeneic bone. RESULTS AND CONCLUSION: The model group was better than the control group in new bone growth and defect repair at 4, 8, 12 weeks after implantation, which was confirmed by general observation, X-ray examination and hematoxylin-eosin staining. There was a large amount of trabecular bone formation and mature lamel ar bone tissue in bone defects of model group by histological observation at 12 weeks after implantation, and bone defects of the model group were repaired basical y; while there were only abundant woven bones in the control group, and bone defects in the control group were not repaired effectively. Scores on Lane-Sandhu’s X-ray combined with histological observation were higher in the model group than the control group (P < 0.05). Biomechanical test showed that the maximum pressure load of the femoral condyle and load/strain ratio in the model group were significantly higher than those in the control group at 12 weeks after implantation (P < 0.05),while the maximum strain and displacement of the model group was lower than that of the control group (P < 0.05). These findings suggest that the combination of bone marrow mesenchymal stem cells and al ogeneic bone is superior to simple al ogeneic bone implantation in the repair of cancel ous bone defects of the femoral condyle.