中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
28期
5204-5208
,共5页
组织构建%组织构建与生物活性因子%神经生长因子%烫伤创面%转化生长因子β1%碱性成纤维细胞因子%创面愈合%大鼠%愈合时间%创面残余率%细胞增殖%细胞DNA周期
組織構建%組織構建與生物活性因子%神經生長因子%燙傷創麵%轉化生長因子β1%堿性成纖維細胞因子%創麵愈閤%大鼠%愈閤時間%創麵殘餘率%細胞增殖%細胞DNA週期
조직구건%조직구건여생물활성인자%신경생장인자%탕상창면%전화생장인자β1%감성성섬유세포인자%창면유합%대서%유합시간%창면잔여솔%세포증식%세포DNA주기
背景：研究证实神经生长因子促进创面组织释放内源性各类生长因子及生长因子受体，起到正性调节作用，促进修复细胞增殖，加速创面愈合，使创面愈合由以往的被动等待自愈发展到主动调控愈合。目的：观察局部应用神经生长因子对大鼠烫伤创面转化生长因子β1和碱性成纤维细胞因子表达的影响。方法：取24只成年 SD 大鼠，于背部制成深Ⅱ度烫伤创面，随机分为4组，烧伤创面清创后，分别予以两层浸湿有1，2.5，5 mg/L 神经生长因子溶液及等渗盐水纱布覆盖。治疗后3，5，9，14 d 观察创面愈合时间和创面残留率，切取创面组织进行组织学观察，检测创面转化生长因子β1，碱性成纤维细胞因子的表达及细胞 DNA 周期的变化。结果与结论：各治疗组创面愈合时间较对照组提前，尤以5 mg/L 神经生长因子治疗组最为明显(P <0.01)，各治疗组创面残留率较对照组明显减小；创面组织学显示治疗组真皮浅层有核细胞数较对照组明显增多；各治疗组给药时间点转化生长因子β1，碱性成纤维细胞因子表达均强于对照组，第5天和第9天表达强于第3天和第14天；各治疗组细胞在 S 期的百分比较对照组明显增加，其中5 mg/L 神经生长因子组增加最为显著(P <0.01)。结果显示局部应用神经生长因子可通过促进创面转化生长因子β1及碱性成纤维细胞因子表达，刺激细胞有丝分裂，促使细胞增殖，加速大鼠烫伤创面愈合。
揹景：研究證實神經生長因子促進創麵組織釋放內源性各類生長因子及生長因子受體，起到正性調節作用，促進脩複細胞增殖，加速創麵愈閤，使創麵愈閤由以往的被動等待自愈髮展到主動調控愈閤。目的：觀察跼部應用神經生長因子對大鼠燙傷創麵轉化生長因子β1和堿性成纖維細胞因子錶達的影響。方法：取24隻成年 SD 大鼠，于揹部製成深Ⅱ度燙傷創麵，隨機分為4組，燒傷創麵清創後，分彆予以兩層浸濕有1，2.5，5 mg/L 神經生長因子溶液及等滲鹽水紗佈覆蓋。治療後3，5，9，14 d 觀察創麵愈閤時間和創麵殘留率，切取創麵組織進行組織學觀察，檢測創麵轉化生長因子β1，堿性成纖維細胞因子的錶達及細胞 DNA 週期的變化。結果與結論：各治療組創麵愈閤時間較對照組提前，尤以5 mg/L 神經生長因子治療組最為明顯(P <0.01)，各治療組創麵殘留率較對照組明顯減小；創麵組織學顯示治療組真皮淺層有覈細胞數較對照組明顯增多；各治療組給藥時間點轉化生長因子β1，堿性成纖維細胞因子錶達均彊于對照組，第5天和第9天錶達彊于第3天和第14天；各治療組細胞在 S 期的百分比較對照組明顯增加，其中5 mg/L 神經生長因子組增加最為顯著(P <0.01)。結果顯示跼部應用神經生長因子可通過促進創麵轉化生長因子β1及堿性成纖維細胞因子錶達，刺激細胞有絲分裂，促使細胞增殖，加速大鼠燙傷創麵愈閤。
배경：연구증실신경생장인자촉진창면조직석방내원성각류생장인자급생장인자수체，기도정성조절작용，촉진수복세포증식，가속창면유합，사창면유합유이왕적피동등대자유발전도주동조공유합。목적：관찰국부응용신경생장인자대대서탕상창면전화생장인자β1화감성성섬유세포인자표체적영향。방법：취24지성년 SD 대서，우배부제성심Ⅱ도탕상창면，수궤분위4조，소상창면청창후，분별여이량층침습유1，2.5，5 mg/L 신경생장인자용액급등삼염수사포복개。치료후3，5，9，14 d 관찰창면유합시간화창면잔류솔，절취창면조직진행조직학관찰，검측창면전화생장인자β1，감성성섬유세포인자적표체급세포 DNA 주기적변화。결과여결론：각치료조창면유합시간교대조조제전，우이5 mg/L 신경생장인자치료조최위명현(P <0.01)，각치료조창면잔류솔교대조조명현감소；창면조직학현시치료조진피천층유핵세포수교대조조명현증다；각치료조급약시간점전화생장인자β1，감성성섬유세포인자표체균강우대조조，제5천화제9천표체강우제3천화제14천；각치료조세포재 S 기적백분비교대조조명현증가，기중5 mg/L 신경생장인자조증가최위현저(P <0.01)。결과현시국부응용신경생장인자가통과촉진창면전화생장인자β1급감성성섬유세포인자표체，자격세포유사분렬，촉사세포증식，가속대서탕상창면유합。
BACKGROUND: Studies have confirmed that nerve growth factor can promote wound tissue to release al kinds of endogenous growth factors and growth factor receptors, which play a positive regulatory role. The nerve growth factor can promote cel proliferation and accelerate wound healing, thus making the wound healing developed from the passive waiting healing to active control healing. OBJECTIVE: To investigate the effects of local application of nerve growth factor on the expressions of transforming growth factor β1 and basic fibroblast growth factor in rat burn wounds. METHODS: Twenty-four adult Sprague Dawley rats were used in the study, and Ⅱ degree deep burn wound was made on the back of rats. Then, these rats were randomly divided into four groups. After burn wound debridement, the wounds were covered with gauzes containing 1, 2.5 and 5 μg/mL nerve growth factor solution and normal saline respectively. At 3, 5, 9 and 14 days after treatment, the wound healing time and percentage of residual wound were observed. Then, wound tissues were cut for histological examination, in order to detect the expressions of transforming growth factor β1 and basic fibroblast growth factor in wounds, as wel as the cel ular DNA cycle. RESULTS AND CONCLUSION: The wound healing time in the treatment groups was shorter than that in the control group, especial y in 5 ug/mL nerve growth factor treatment group (P < 0.01), and the percentage of residual wound in the treatment groups was less than that in the control group. The histological examination showed the number of nucleated cells in the superficial dermis of the treatment groups was significant increased when compared with that in the control group; the expressions of transforming growth factor β1 and basic fibroblast growth factor in the treatment groups at different time points were stronger than those in the control group, and the expressions at 5 and 9 days were stronger than those at 3 and 14 days; percentage of cells in S phase of the treatment groups was significantly increased, especial y in 5 mg/L nerve growth factor group (P < 0.01). The results indicate that local application of nerve growth factor can accelerate wound healing by increasing the expressions of transforming growth factor β1 and basic fibroblast growth factor, stimulating mitosis and promoting proliferation.
