CAJ | 학술논문

　　背景：糖尿病是最常见的系统性疾病之一，常引起颌骨及全身其他骨骼结构的变化，以及矿物代谢的异常改变。目的：观察1型糖尿病小鼠下颌骨三维结构以及组织病理学的改变。方法：将小鼠随机分为对照组和糖尿病组，糖尿病组按50 mg/kg 剂量连续5 d 腹腔内注射链脲佐菌素建立1型糖尿病小鼠模型，对照组腹腔注射枸橼酸盐缓冲液。结果与结论：造模3周后，运用显微 CT 技术观察两组小鼠下颌骨三维结构，对其松质骨及皮质骨骨微结构进行定量分析显示，与对照组比较，1型糖尿病小鼠下颌骨感兴趣区域内松质骨骨密度、骨体积分数、骨小梁数量及骨小梁厚度均显著减少(P <0.01，P <0.05)，结构模型指数明显增大(P <0.05)；皮质骨骨密度、皮质骨面积减少(P <0.05)。苏木精-伊红染色显示，1型糖尿病小鼠脱钙后下颌骨松质骨骨小梁数量及体积均变小。说明链脲佐菌素诱导的1型糖尿病小鼠下颌骨松质骨及皮质骨三维结构明显改变，且松质骨骨组织微结构改变更显著。
　　배경：당뇨병시최상견적계통성질병지일，상인기합골급전신기타골격결구적변화，이급광물대사적이상개변。목적：관찰1형당뇨병소서하합골삼유결구이급조직병이학적개변。방법：장소서수궤분위대조조화당뇨병조，당뇨병조안50 mg/kg 제량련속5 d 복강내주사련뇨좌균소건립1형당뇨병소서모형，대조조복강주사구연산염완충액。결과여결론：조모3주후，운용현미 CT 기술관찰량조소서하합골삼유결구，대기송질골급피질골골미결구진행정량분석현시，여대조조비교，1형당뇨병소서하합골감흥취구역내송질골골밀도、골체적분수、골소량수량급골소량후도균현저감소(P <0.01，P <0.05)，결구모형지수명현증대(P <0.05)；피질골골밀도、피질골면적감소(P <0.05)。소목정-이홍염색현시，1형당뇨병소서탈개후하합골송질골골소량수량급체적균변소。설명련뇨좌균소유도적1형당뇨병소서하합골송질골급피질골삼유결구명현개변，차송질골골조직미결구개변경현저。
BACKGROUND: Diabetes mel itus is one of the most common systemic diseases, which often leads to the changes of the jaw and other bone structure, as wel as the abnormal changes of mineral metabolism. OBJECTIVE: To observe the three-dimensional structure and histopathological changes of the mandible in type 1 diabetes mel itus mice. METHODS: The mice were randomly divided into control group and diabetes mel itus group. The diabetes mel itus group received intraperitoneal injection of 50 mg/kg streptozotocin for 5 days to establish a type 1 diabetes mel itus model, and the control group received intraperitoneal injection of citrate buffer. RESULTS AND CONCLUSION: At 3 weeks after modeling, the micro-CT technique was used to observe the three-dimensional structure of the mandibles in the two groups. The quantitative analysis on the microstructure of cancel ous bone and cortical bone showed that the bone mineral density, bone volume fraction, trabecular number and trabecular thickness of cancel ous bone in the interest region in the mandible of type 1 diabetes mel itus mice were significantly decreased when compared with that in the control group (P < 0.01, P < 0.05), while the structure model index was increased significantly (P < 0.05); the mineral density and area of cortical bone were decreased in the diabetes mel itus group (P < 0.05). Hematoxylin-eosin staining showed that the number and volume of mandibular trabeculae of type 1 diabetes mel itus mice were decreased. The results suggest that the three-dimensional structure of the cancel ous bone and cortical bone in the streptozotocin-induced type 1 diabetes mel itus mice are changed significantly, and the microstructure change of the cancel ous bone is more obvious.