CAJ | 학술논문

　　背景：牙周组织工程技术为修复牙周炎骨组织缺损提供新思路和新方法。目的：体外培养小型猪牙周膜干细胞，并与羟基磷灰石进行骨组织构建，研究其与羟基磷灰石支架材料的生物相容性。方法：采用组织块法获得小型猪牙周膜干细胞，用免疫荧光法检测细胞中基质细胞抗原1的表达。取第3代小型猪牙周膜干细胞接种在羟基磷灰石支架材料上复合培养，分别在培养第1，3，7天用扫描电镜观察细胞在羟基磷灰石上的生长情况。结果与结论：原代培养的小型猪牙周膜干细胞生长良好，细胞中基质细胞抗原1免疫荧光呈阳性。扫描电镜结果表明，培养第1，3，7天，牙周膜干细胞在羟基磷灰石上生长良好。证实，实验采用组织块法成功体外分离培养了小型猪牙周膜干细胞，可在羟基磷灰石支架材料上良好的生长。
　　배경：아주조직공정기술위수복아주염골조직결손제공신사로화신방법。목적：체외배양소형저아주막간세포，병여간기린회석진행골조직구건，연구기여간기린회석지가재료적생물상용성。방법：채용조직괴법획득소형저아주막간세포，용면역형광법검측세포중기질세포항원1적표체。취제3대소형저아주막간세포접충재간기린회석지가재료상복합배양，분별재배양제1，3，7천용소묘전경관찰세포재간기린회석상적생장정황。결과여결론：원대배양적소형저아주막간세포생장량호，세포중기질세포항원1면역형광정양성。소묘전경결과표명，배양제1，3，7천，아주막간세포재간기린회석상생장량호。증실，실험채용조직괴법성공체외분리배양료소형저아주막간세포，가재간기린회석지가재료상량호적생장。
BACKGROUND:Periodontal tissue engineering technology provides new ideas and new ways for periodontitis-induced bone defect repair. OBJECTIVE:To develop a culture model for the periodontal ligament cells of miniature swine, which was constructed with hydroxyapatite, to investigate the biocompatibility with hydroxyapatite. METHODS:Periodontal ligament cells from miniature swine were harvested by using tissue explant method. Immunofluorescence was used to detect the expression of stromal cel antigen 1 in the periodontal ligament cells of miniature swine. The third passage cells were co-cultured with a three-dimension hydroxyapatite scaffold, and the biological characteristics of the cells were observed under a scanning electron microscope at days 1, 3, 7 of co-culture. RESULTS AND CONCLUSION:The pirmary miniature swine periodontal ligament cells grew wel , and they were positive for stromal cel antigen 1. Under the scanning electron microscope, the periodontal ligament cells of miniature swine grew wel on the hydroxyapatite scaffold at days 1, 3, 7 of co-culture. These prove that the miniature swine periodontal ligament cells, which can be separated using tissue explant method and cultured successful y in vitro, can grow wel on the hydroxyapatite scaffold.