CAJ | 학술논문

生物材料%组织工程骨材料%异种骨支架材料%羟基磷灰石生物陶瓷%生物相容性%皮下植入%细胞毒性%骨髓间充质干细胞%国家自然科学基金生物材料%組織工程骨材料%異種骨支架材料%羥基燐灰石生物陶瓷%生物相容性%皮下植入%細胞毒性%骨髓間充質榦細胞%國傢自然科學基金
생물재료%조직공정골재료%이충골지가재료%간기린회석생물도자%생물상용성%피하식입%세포독성%골수간충질간세포%국가자연과학기금
biomaterials%tissue-engineered bone materials%xenogenic bone scaffold materials%hydroxyapatite biological ceramic%biocompatibility%subcutaneous implantation%cytotoxicity%bone marrow mesenchymal stem cells%National Natural Science Foundation of China

　　背景：经过一系列理化处理去抗原后的异种骨支架材料，其形态与结构类似于人体骨组织。目的：检测3种不同方法制备去抗原异种骨支架材料的生物相容性。方法：将经过物理、化学及物理联合化学方法制备的去抗原异种骨支架与羟基磷灰石生物陶瓷4组材料分别植入裸鼠背部皮下组织，术后4，8，12周行组织学观察。以经过物理、化学及物理联合化学方法制备的去抗原异种骨支架材料浸提液分别培养羊骨髓间充质干细胞7 d，观察细胞黏附、生长、增殖及基质分泌情况。结果与结论：术后4周时，4组材料周围均有较强的炎症反应；术后12周时，物理及物理联合化学制备的去抗原异种骨支架和羟基磷灰石生物陶瓷材料孔隙内及周围组织炎症反应基本消失，极少量炎症细胞存在，材料降解较术后8周时多。化学制备的去抗原异种骨支架材料孔隙内及周围组织炎症反应仍存在；表明物理及物理联合化学制备的去抗原异种骨支架具有良好的组织相容性。羟基磷灰石生物陶瓷和物理联合化学法制备的去抗原异种骨支架材料周围有少量成骨细胞规则排列，并可见少量成骨，表明有异位成骨的趋势。经过物理或物理联合化学方法去除抗原的异种骨支架材料具有良好的细胞相容性；单纯经过化学方法处理的异种骨材料细胞相容性较差，不符合生物材料安全性标准。
　　배경：경과일계렬이화처리거항원후적이충골지가재료，기형태여결구유사우인체골조직。목적：검측3충불동방법제비거항원이충골지가재료적생물상용성。방법：장경과물리、화학급물리연합화학방법제비적거항원이충골지가여간기린회석생물도자4조재료분별식입라서배부피하조직，술후4，8，12주행조직학관찰。이경과물리、화학급물리연합화학방법제비적거항원이충골지가재료침제액분별배양양골수간충질간세포7 d，관찰세포점부、생장、증식급기질분비정황。결과여결론：술후4주시，4조재료주위균유교강적염증반응；술후12주시，물리급물리연합화학제비적거항원이충골지가화간기린회석생물도자재료공극내급주위조직염증반응기본소실，겁소량염증세포존재，재료강해교술후8주시다。화학제비적거항원이충골지가재료공극내급주위조직염증반응잉존재；표명물리급물리연합화학제비적거항원이충골지가구유량호적조직상용성。간기린회석생물도자화물리연합화학법제비적거항원이충골지가재료주위유소량성골세포규칙배렬，병가견소량성골，표명유이위성골적추세。경과물리혹물리연합화학방법거제항원적이충골지가재료구유량호적세포상용성；단순경과화학방법처리적이충골재료세포상용성교차，불부합생물재료안전성표준。
BACKGROUND:The form and structure of antigen-extracted xenogeneic cancel ous bone through series of physical and chemical treatment are similar to human tissue. OBJECTIVE:To detect the biocompatibility of antigen-extracted xenogeneic cancel ous bone matrix prepared by three different ways. METHODS:The antigen-extracted xenogeneic cancel ous bone scaffold materials which were prepared through physical, chemical and physical-chemical combined methods and hydroxy apatite biological ceramic materials were implanted into the dorsum subcutaneous tissue. Histological observation was done at 4, 8 and 12 weeks after surgery. The antigen-extracted xenogeneic cancel ous bone scaffold materials which were prepared through physical, chemical and physical-chemical combined methods respectively was used to culture sheep bone marrow mesenchymal stem cells for 7 days. Cel adhesion, growth, proliferation and stroma secretion were observed. RESULTS AND CONCLUSION:At 4 weeks after surgery, a strong inflammatory reaction was detected around materials in four groups. At 12 weeks, the xenogeneic bone materials prepared through physical and physical-chemical combined methods and hydroxy apatite biological ceramic materials internal pore and surrounding tissue inflammation disappeared basical y, with the presence of thimbleful inflammation cells. The material degradation was more than at 8 weeks. The xenogeneic bone materials prepared through chemical methods material internal pore and surrounding tissue inflammation stil existed, suggesting that the xenogeneic bone materials prepared through physical and physical-chemical combined methods exhibited good histocompatibility. A smal amount of orderly osteoblasts existed around hydroxy apatite biological ceramic materials and physical-chemical prepared materials, with a smal amount of bone. These suggested that there was a tendency for ectopic bone formation. The xenogeneic cancel ous bone materials prepared through physical or physical-chemical combined methods have better cytocompatibility. However, scaffold materials prepared through chemical method have poor cytocompatibility and they are not qualified for the safety standards of biological materials.