CAJ | 학술논문

　　背景：药物是目前糖尿病治疗最主要的方法，但病情的进展以及相关并发症的发生使药物的作用受到挑战。目的：探讨骨髓间充质干细胞与胰岛细胞共移植治疗糖尿病的应用效果以及可行性。方法：分离、纯化大鼠骨髓间充质干细胞并进行体外培养，建立糖尿病模型，对糖尿病模型大鼠分别注射骨髓间充质干细胞、骨髓间充质干细胞和胰岛细胞共培养混合物以及生理盐水或磷酸盐缓冲液作为对照。通过观察监测各糖尿病模型大鼠血糖水平的变化、胰岛素分泌情况以及胰腺组织的病理学变化评估移植治疗效果。结果与结论：骨髓间充质干细胞移植治疗糖尿病模型大鼠，移植后C-肽值明显升高，血糖水平明显下降，但仍未降至正常范围内，且随着时间的延长，血糖水平再次升高。骨髓间充质干细胞与胰岛细胞共移植治疗糖尿病模型大鼠，血糖水平亦明显下降，且下降程度大于单纯骨髓间充质干细胞移植治疗，可下降至正常水平，并在一定时间内能够维持。骨髓间充质干细胞与胰岛细胞共移植治疗糖尿病具有一定的效果，具有应用的可行性。
　　배경：약물시목전당뇨병치료최주요적방법，단병정적진전이급상관병발증적발생사약물적작용수도도전。목적：탐토골수간충질간세포여이도세포공이식치료당뇨병적응용효과이급가행성。방법：분리、순화대서골수간충질간세포병진행체외배양，건립당뇨병모형，대당뇨병모형대서분별주사골수간충질간세포、골수간충질간세포화이도세포공배양혼합물이급생리염수혹린산염완충액작위대조。통과관찰감측각당뇨병모형대서혈당수평적변화、이도소분비정황이급이선조직적병이학변화평고이식치료효과。결과여결론：골수간충질간세포이식치료당뇨병모형대서，이식후C-태치명현승고，혈당수평명현하강，단잉미강지정상범위내，차수착시간적연장，혈당수평재차승고。골수간충질간세포여이도세포공이식치료당뇨병모형대서，혈당수평역명현하강，차하강정도대우단순골수간충질간세포이식치료，가하강지정상수평，병재일정시간내능구유지。골수간충질간세포여이도세포공이식치료당뇨병구유일정적효과，구유응용적가행성。
BACKGROUND:Drug treatment is the main method for the treatment of diabetes currently, but the development of the disease and occurrence of related complications are the chal enges to the effect of drugs. OBJECTIVE:To investigate the effect and feasibility of co-transplantation of bone marrow mesenchymal stem cells and islet cells for the treatment of diabetes. METHODS:The rat bone marrow mesenchymal stem cells were separated and purified, and cultured in vitro to establish the diabetes models. The rat diabetes models were injected with bone marrow mesenchymal stem cells, co-cultured mixture of bone marrow mesenchymal stem cells and islet cells, and normal saline or phosphate buffer (control). The effect of transplantation was evaluated through observing the blood glucose levels, insulin secretion, and pathological changes of pancreatic tissue in the rat diabetes models. RESUTLS AND CONCLUSION:In the diabetes rats treated with bone marrow mesenchymal stem cel transplantation, the C-peptide levels were significantly increased after transplantation, while the blood glucose levels were significantly decreased, but not lower than the normal level, and the blood glucose levels were increased again with the time prolonging. In the diabetes rats treated with co-transplantation of bone marrow mesenchymal stem cells and islet cells, the blood glucose levels were decreased significantly and lower than the normal level which was maintained in a certain time, and the decreasing degree was larger than that in the rats treated with simple bone marrow mesenchymal stem cel transplantation. Co-transplantation of bone marrow mesenchymal stem cells and islet cells is feasible for the treatment of diabetes with a certain effect.