CAJ | 학술논문

器官移植%器官移植学术探讨%骨髓间充质干细胞%诱导分化%共同培养%胰岛细胞%细胞移植%糖尿病%免疫细胞化学%双硫腙染色%其他基金器官移植%器官移植學術探討%骨髓間充質榦細胞%誘導分化%共同培養%胰島細胞%細胞移植%糖尿病%免疫細胞化學%雙硫腙染色%其他基金
기관이식%기관이식학술탐토%골수간충질간세포%유도분화%공동배양%이도세포%세포이식%당뇨병%면역세포화학%쌍류종염색%기타기금
organ transplantation%organ transplantation academic discussion%bone marrow mesenchymal stem cells%induced differentiation%co-culture%islet cells%cell transplantation%diabetes%immunocytochemistry%dithizone staining%other grants-supported paper

　　背景：移植胰岛及胰岛细胞治疗糖尿病已初见成效，但由于胰岛来源匮乏和免疫排斥反应而研究受阻。目的：移植将大鼠骨髓间充质干细胞在体外诱导分化为胰岛样细胞，观察其对糖尿病大鼠的治疗作用。方法：将大鼠骨髓间充质干细胞用碱性成纤维细胞生长因子、肝细胞生长因子等诱导，免疫细胞染色等检测诱导情况。SD 大鼠腹腔注射链脲佐菌素建立糖尿病模型，建模成功后，随机分为对照组移植诱导胰岛样细胞的实验组，实验组经肾包囊移植诱导后的胰岛样细胞，对照组移植相同体积生理盐水，观察移植后糖尿病大鼠血糖和体质量变化。结果与结论：大鼠骨髓间充质干细胞体外经肝细胞生长因子、碱性成纤维细胞生长因子等因子诱导后可以向胰岛样细胞转化。细胞移植后，对照组大鼠血糖无明显变化(P>0.05)，实验组大鼠血糖与对照组和移植前相比较，明显降低(P<0.05)。大鼠骨髓间充质干细胞经含肝细胞生长因子、碱性成纤维细胞生长因子等的诱导体系可诱导成胰岛样细胞，经诱导的细胞有一定胰岛素分泌能力，将诱导后细胞通过肾包囊途径移植入糖尿病大鼠体内，可降低大鼠血糖水平。
　　배경：이식이도급이도세포치료당뇨병이초견성효，단유우이도래원궤핍화면역배척반응이연구수조。목적：이식장대서골수간충질간세포재체외유도분화위이도양세포，관찰기대당뇨병대서적치료작용。방법：장대서골수간충질간세포용감성성섬유세포생장인자、간세포생장인자등유도，면역세포염색등검측유도정황。SD 대서복강주사련뇨좌균소건립당뇨병모형，건모성공후，수궤분위대조조이식유도이도양세포적실험조，실험조경신포낭이식유도후적이도양세포，대조조이식상동체적생리염수，관찰이식후당뇨병대서혈당화체질량변화。결과여결론：대서골수간충질간세포체외경간세포생장인자、감성성섬유세포생장인자등인자유도후가이향이도양세포전화。세포이식후，대조조대서혈당무명현변화(P>0.05)，실험조대서혈당여대조조화이식전상비교，명현강저(P<0.05)。대서골수간충질간세포경함간세포생장인자、감성성섬유세포생장인자등적유도체계가유도성이도양세포，경유도적세포유일정이도소분비능력，장유도후세포통과신포낭도경이식입당뇨병대서체내，가강저대서혈당수평。
BACKGROUND:Islet and islet cel transplantation for the treatment of diabetes has achieved effect, but the research is limited dut to the shortage of islet and immune rejection. OBJECTIVE:To observe the effect of transplantation of islet-like cells that in vitro differentiated from bone marrow mesenchymal stem cells on the treatment of diabetes in rats. METHODS:The rat bone marrow mesenchymal stem cells were induced with basic fibroblast growth factors and hepatocyte growth factors, and then received immunocytochemistry staining to detect the induction. The Sprague Dawley rats received intraperitoneal injection of streptozotocin to establish the diabetes models. After modeling, the rats were randomly divided into control group and experimental group (transplanted with induced islet-like cells). The experimental group was transplanted with the induced islet-like cells through renal capsule, and the control group was transplanted with normal saline in the same dose. The blood glucose and body mass of the diabetes rats were observed after transplantation. RESULTS AND CONCLUSION:The bone marrow mesenchymal stem cells could differentiate into islet-like cells after in vitro induced with basic fibroblast growth factors and hepatocyte growth factors. There was no significant change in blood glucose of the control group after transplantation (P>0.05), and the blood glucose of the rats in the experimental group was significantly decreased compared with the control group (P<0.05). The bone marrow mesenchymal stem cells can differentiate into islet-like cells after in vitro induced with the induction system containing basic fibroblast growth factors and hepatocyte growth factors, and the islet-like cells have a certain ability of insulin secretion. The transplantation of induced islet-like cells after transplanted into the diabetes rats through renal capsule can decrease the blood glucose level of the rats.