CAJ | 학술논문

　　背景：建立大鼠原位肺移植模型是研究肺移植后慢性排异反应的关键，但因其需要精细外科技术，难度较大，限制了这一模型的应用。目的：改良麻醉和肺移植过程，建立快速、安全、具有可重复性的大鼠肺移植模型。方法：共完成42只大鼠动物实验，供体受体各21只。采用胸骨正中切口解剖供体左肺并置入套管(用静脉留置针套管剪取1.5 mm长制成)获取大鼠供肺。大鼠受体经左侧开胸，利用套管技术吻合完成供肺植入。记录并计算手术时间及移植成功率。结果与结论：移植后大鼠均存活，大鼠供肺获取时间平均为(35.3±5.1) min；供肺套管时间平均为(12.5±4.6) min；大鼠受体肺移植时间平均为(50.2±3.3) min；吻合时间平均为(27.7±6.2) min。开放肺动、静脉血流后，全肺迅速变红，血流灌注充分，静脉回流通畅；恢复机械通气后，所有移植肺膨胀良好。结果证实，改良后的麻醉和肺移植技术可为大鼠肺移植的免疫与排异研究提供稳定、可靠、可重复的动物模型。
　　배경：건립대서원위폐이식모형시연구폐이식후만성배이반응적관건，단인기수요정세외과기술，난도교대，한제료저일모형적응용。목적：개량마취화폐이식과정，건립쾌속、안전、구유가중복성적대서폐이식모형。방법：공완성42지대서동물실험，공체수체각21지。채용흉골정중절구해부공체좌폐병치입투관(용정맥류치침투관전취1.5 mm장제성)획취대서공폐。대서수체경좌측개흉，이용투관기술문합완성공폐식입。기록병계산수술시간급이식성공솔。결과여결론：이식후대서균존활，대서공폐획취시간평균위(35.3±5.1) min；공폐투관시간평균위(12.5±4.6) min；대서수체폐이식시간평균위(50.2±3.3) min；문합시간평균위(27.7±6.2) min。개방폐동、정맥혈류후，전폐신속변홍，혈류관주충분，정맥회류통창；회복궤계통기후，소유이식폐팽창량호。결과증실，개량후적마취화폐이식기술가위대서폐이식적면역여배이연구제공은정、가고、가중복적동물모형。
BACKGROUND:Orthotopic lung transplantation model in a rat is the key to investigate the chronic rejection after lung transplantation. However, the precise surgical technique and difficult operation limit the application of the model. OBJECTIVE:To improve the process of anesthesia and lung transplantation, and to establish a rapid, safe and reversible rat lung transplantation model. METHODS:A total of 42 rats were used to establish the model, including 21 donor models and 21 receptor models. The donor lung was excised by median sternotomy with dissection of the left lung and implantation of cuffs (intravenous catheters cut into 1.5 mm sections). The left lung was implanted in the recipient by lateral thoracotomy using the cuffs for anastomoses. The duration of surgery and success rate of transplantation were recorded and calculated. RESULTS AND CONCLUSION:The survival rate of rats after lung transplantation was 100%. The time of left donor lung extraction was (35.3±5.1) minutes in average. The time of placing cuff in donor lung was (12.5±4.6) minutes in average. The surgical procedure time of recipient was (50.2±3.3) minutes. The time of arteriovenous and bronchus casing anastomosis was (27.7±6.2) minutes. After pulmonary artery and vein blood flow was disparked, the whole lung turned red rapidly, blood perfusion was sufficient, venous returned unimpeded;after mechanical ventilation resumed, al graft lungs expanded wel . This improved anesthesia and lung transplantation technique in rats can provide a valid, reliable and reproducible animal model for studying immune responses and rejection in lung transplantation.