中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
30期
5511-5517
,共7页
詹玉林%安智全%孙鲁源%张长青%曾炳芳%许锋%侯国柱%李文举%朱小萌%宋兴华
詹玉林%安智全%孫魯源%張長青%曾炳芳%許鋒%侯國柱%李文舉%硃小萌%宋興華
첨옥림%안지전%손로원%장장청%증병방%허봉%후국주%리문거%주소맹%송흥화
骨关节植入物%骨关节损伤基础实验%骨缺损%骨形态发生蛋白2%长度%骨愈合%兔%桡骨%国家自然科学基金
骨關節植入物%骨關節損傷基礎實驗%骨缺損%骨形態髮生蛋白2%長度%骨愈閤%兔%橈骨%國傢自然科學基金
골관절식입물%골관절손상기출실험%골결손%골형태발생단백2%장도%골유합%토%뇨골%국가자연과학기금
bone and joint implants%basic experiment of bone joint injury%bone defect%bone morphogenetic protein 2%length%bone healing%rabbits%radius bone%National Natural Science Foundation of China
背景：有研究表明在骨缺损的状态下，骨形态发生蛋白2的分布存在一定的规律。目的：观察不同长度兔桡骨骨缺损部位骨形态发生蛋白2的表达。方法：将48只新西兰大白兔随机分成2组，分别在麻醉下用线锯造成兔左前臂桡骨中段0.5和3.0 cm的骨缺损。结果与结论：Western blot检测显示，0.5 cm骨缺损组骨缺损部位骨形态发生蛋白2蛋白表达量在损伤后1，3，4周呈逐渐增多的趋势，且每组均较损伤即刻明显增加(P <0.05)；3.0 cm骨缺损组骨缺损部位的骨形态发生蛋白2的相对表达量在损伤后3周达到高峰(P<0.05)，其峰值水平明显高于0.5 cm骨缺损组(P<0.05)，基本维持在较高水平。对骨痂的生成情况进行量化评估结果显示，损伤后3，4周，3.0 cm骨缺损组的骨痂生成量较0.5 cm骨缺损组明显减少(P<0.05)。证实，兔桡骨3.0 cm的骨缺损部位骨形态发生蛋白2表达明显上调，但还不能使该长度的骨缺损自行愈合。
揹景：有研究錶明在骨缺損的狀態下，骨形態髮生蛋白2的分佈存在一定的規律。目的：觀察不同長度兔橈骨骨缺損部位骨形態髮生蛋白2的錶達。方法：將48隻新西蘭大白兔隨機分成2組，分彆在痳醉下用線鋸造成兔左前臂橈骨中段0.5和3.0 cm的骨缺損。結果與結論：Western blot檢測顯示，0.5 cm骨缺損組骨缺損部位骨形態髮生蛋白2蛋白錶達量在損傷後1，3，4週呈逐漸增多的趨勢，且每組均較損傷即刻明顯增加(P <0.05)；3.0 cm骨缺損組骨缺損部位的骨形態髮生蛋白2的相對錶達量在損傷後3週達到高峰(P<0.05)，其峰值水平明顯高于0.5 cm骨缺損組(P<0.05)，基本維持在較高水平。對骨痂的生成情況進行量化評估結果顯示，損傷後3，4週，3.0 cm骨缺損組的骨痂生成量較0.5 cm骨缺損組明顯減少(P<0.05)。證實，兔橈骨3.0 cm的骨缺損部位骨形態髮生蛋白2錶達明顯上調，但還不能使該長度的骨缺損自行愈閤。
배경：유연구표명재골결손적상태하，골형태발생단백2적분포존재일정적규률。목적：관찰불동장도토뇨골골결손부위골형태발생단백2적표체。방법：장48지신서란대백토수궤분성2조，분별재마취하용선거조성토좌전비뇨골중단0.5화3.0 cm적골결손。결과여결론：Western blot검측현시，0.5 cm골결손조골결손부위골형태발생단백2단백표체량재손상후1，3，4주정축점증다적추세，차매조균교손상즉각명현증가(P <0.05)；3.0 cm골결손조골결손부위적골형태발생단백2적상대표체량재손상후3주체도고봉(P<0.05)，기봉치수평명현고우0.5 cm골결손조(P<0.05)，기본유지재교고수평。대골가적생성정황진행양화평고결과현시，손상후3，4주，3.0 cm골결손조적골가생성량교0.5 cm골결손조명현감소(P<0.05)。증실，토뇨골3.0 cm적골결손부위골형태발생단백2표체명현상조，단환불능사해장도적골결손자행유합。
BACKGROUND:It has been studied that the distribution of bone morphogenetic protein 2 is regular under bone defect situation. OBJECTIVE:To observe the expression of bone morphogenetic protein 2 in rabbit radial defect site with different lengths. METHODS:Forty-eight New Zealand rabbits were divided into two groups randomly, 0.5 cm bone defect and 3.0 cm bone defect were made by wire saw at the middle part of radius bone after anaesthesia. RESULTS AND CONCLUSION:Western blot results showed that in the 0.5 cm bone defect group, the expression of bone morphogenetic protein 2 of the tissues in the bone defect site was increased gradual y at 1, 3, 4 weeks after operation, and the expression in each defect group was increased when compared with that immediately after injury (P<0.05). In the 3.0 cm bone defect group, the expression of bone morphogenetic protein 2 of tissues in bone defect site was increased gradual y and reached to its peak at 3 weeks after the operation (P<0.05), and the peak value in the 3.0 cm bone defect group was significantly higher than that in 0.5 cm bone defect group (P<0.05). The peak value was maintained in high level. The comparison of bone cal us formation showed that the bone cal us formation of 3.0 cm bone defect group was less than that of the 0.5 cm bone defect group at 3 and 4 weeks after operation (P<0.05). The results indicate that expression of the bone morphogenetic protein 2 in 3.0 cm bone defect site is increased significantly, but the expression level cannot make the bone defect heal itself.
