中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
30期
5503-5510
,共8页
郭超峰%张宏其%高琪乐%唐明星%刘少华%邓盎%王昱翔%鲁世金%李劲松%尹新华
郭超峰%張宏其%高琪樂%唐明星%劉少華%鄧盎%王昱翔%魯世金%李勁鬆%尹新華
곽초봉%장굉기%고기악%당명성%류소화%산앙%왕욱상%로세금%리경송%윤신화
骨关节植入物%脊柱损伤基础实验%细胞因子%血清单核细胞趋化蛋白1%基因多态性%脊柱结核%易感性%炎症反应%诊断%其他基金
骨關節植入物%脊柱損傷基礎實驗%細胞因子%血清單覈細胞趨化蛋白1%基因多態性%脊柱結覈%易感性%炎癥反應%診斷%其他基金
골관절식입물%척주손상기출실험%세포인자%혈청단핵세포추화단백1%기인다태성%척주결핵%역감성%염증반응%진단%기타기금
bone and joint implants%basic experiment of spinal cord injury%cytokines%serum monocyte chemoattactant protein-1%gene polymorphism%spinal tuberculosis%susceptibility%inflammatory response%diagnosis%other grants-supported paper
背景：研究表明单核细胞趋化蛋白1基因多态性与脊柱结核易感性相关。目的：探讨湖南汉族人群血清趋化因子单核细胞趋化蛋白1表达水平与脊柱结核易感性的关系。方法：连续纳入2004年12月至2010年12月中南大学湘雅医院收治的湖南汉族新发脊柱结核患者及健康志愿者，抽取受试者清晨空腹外周静脉血2 mL，应用PCR和DNA直接测序法检测受试者单核细胞趋化蛋白1-362位点的基因型，应用酶联免疫吸附试验检测受试者血清单核细胞趋化蛋白1水平。运用ROC曲线进行诊断性检验，计算血清单核细胞趋化蛋白1水平对脊柱结核的诊断阈值并分析诊断效价。结果与结论：共纳入符合条件的脊柱结核患者208例，健康志愿者210名。脊柱结核患者血清单核细胞趋化蛋白1水平显著高于健康志愿者[(134.58±51.63) ng/L vs.(39.18±17.45) ng/L，P<0.05]。且血清单核细胞趋化蛋白1水平不受性别影响，但在单核细胞趋化蛋白1-362-CC基因型人群中表达显著增高。当受试者血清单核细胞趋化蛋白1大于101.65 ng/L时，提示受试者可能已经罹患脊柱结核(敏感度：85.5%，特异度：94.3%，约登指数：0.799，ROC曲线下面积：0.946，95%可信区间：0.916-0.975，P <0.01)。可见在湖南汉族人群中，血清单核细胞趋化蛋白1水平与脊柱结核的易感性相关，高表达的血清单核细胞趋化蛋白1可作为诊断脊柱结核的指标之一。
揹景：研究錶明單覈細胞趨化蛋白1基因多態性與脊柱結覈易感性相關。目的：探討湖南漢族人群血清趨化因子單覈細胞趨化蛋白1錶達水平與脊柱結覈易感性的關繫。方法：連續納入2004年12月至2010年12月中南大學湘雅醫院收治的湖南漢族新髮脊柱結覈患者及健康誌願者，抽取受試者清晨空腹外週靜脈血2 mL，應用PCR和DNA直接測序法檢測受試者單覈細胞趨化蛋白1-362位點的基因型，應用酶聯免疫吸附試驗檢測受試者血清單覈細胞趨化蛋白1水平。運用ROC麯線進行診斷性檢驗，計算血清單覈細胞趨化蛋白1水平對脊柱結覈的診斷閾值併分析診斷效價。結果與結論：共納入符閤條件的脊柱結覈患者208例，健康誌願者210名。脊柱結覈患者血清單覈細胞趨化蛋白1水平顯著高于健康誌願者[(134.58±51.63) ng/L vs.(39.18±17.45) ng/L，P<0.05]。且血清單覈細胞趨化蛋白1水平不受性彆影響，但在單覈細胞趨化蛋白1-362-CC基因型人群中錶達顯著增高。噹受試者血清單覈細胞趨化蛋白1大于101.65 ng/L時，提示受試者可能已經罹患脊柱結覈(敏感度：85.5%，特異度：94.3%，約登指數：0.799，ROC麯線下麵積：0.946，95%可信區間：0.916-0.975，P <0.01)。可見在湖南漢族人群中，血清單覈細胞趨化蛋白1水平與脊柱結覈的易感性相關，高錶達的血清單覈細胞趨化蛋白1可作為診斷脊柱結覈的指標之一。
배경：연구표명단핵세포추화단백1기인다태성여척주결핵역감성상관。목적：탐토호남한족인군혈청추화인자단핵세포추화단백1표체수평여척주결핵역감성적관계。방법：련속납입2004년12월지2010년12월중남대학상아의원수치적호남한족신발척주결핵환자급건강지원자，추취수시자청신공복외주정맥혈2 mL，응용PCR화DNA직접측서법검측수시자단핵세포추화단백1-362위점적기인형，응용매련면역흡부시험검측수시자혈청단핵세포추화단백1수평。운용ROC곡선진행진단성검험，계산혈청단핵세포추화단백1수평대척주결핵적진단역치병분석진단효개。결과여결론：공납입부합조건적척주결핵환자208례，건강지원자210명。척주결핵환자혈청단핵세포추화단백1수평현저고우건강지원자[(134.58±51.63) ng/L vs.(39.18±17.45) ng/L，P<0.05]。차혈청단핵세포추화단백1수평불수성별영향，단재단핵세포추화단백1-362-CC기인형인군중표체현저증고。당수시자혈청단핵세포추화단백1대우101.65 ng/L시，제시수시자가능이경리환척주결핵(민감도：85.5%，특이도：94.3%，약등지수：0.799，ROC곡선하면적：0.946，95%가신구간：0.916-0.975，P <0.01)。가견재호남한족인군중，혈청단핵세포추화단백1수평여척주결핵적역감성상관，고표체적혈청단핵세포추화단백1가작위진단척주결핵적지표지일。
BACKGROUND:The monocyte chemoattactant protein-1 gene polymorphism is associated with spinal tuberculosis susceptibility. OBJECTIVE:To investigate the association between serum monocyte chemoattactant protein-1 expression level and spinal tuberculosis susceptibility in Han population of Hunan province. METHODS:The patients with spinal tuberculosis and the healthy volunteers were recruited in Xiangya Hospital of Central South University from December 2004 to December 2010. The empty peripheral venous blood 2 mL were col ected from the subjects in early morning, then the monocyte chemoattactant protein-1-362 genotypes were detected by polymerase chain reaction and DNA sequencing technology. And the serum monocyte chemoattactant protein-1 level was detected by enzyme linked immunosorbent assay technology. The ROC curve was used for diagnostic tests to calculate diagnostic threshold value of serum monocyte chemoattactant protein-1 level to spinal tuberculosis susceptibility, and to analyze the diagnostic titer. RESULTS AND CONCLUSION:208 patients with spinal tuberculosis and 210 healthy volunteers were included. The serum monocyte chemoattactant protein-1 level of the spinal tuberculosis patients was significantly higher than that of the healthy volunteers [(134.58±51.63) ng/L vs. (39.18±17.45) ng/L, P<0.01]. The serum monocyte chemoattactant protein-1 level could not be affected by gender, but over-expressed in patients with monocyte chemoattactant protein-1-362-CC genotypes. The serum monocyte chemoattactant protein-1 level higher than 101.65 ng/L indicated that the patients might suffered from spinal tuberculosis (sensitivity:85.5%, specificity:94.3%, Youden index:0.799, area under curve of ROC:0.946, 95%confidence interval:0.916-0.975, P<0.01). The serum monocyte chemoattactant protein-1 level may be associated with spinal tuberculosis susceptibility in Han population of Hunan province, highly expressed serum monocyte chemoattactant protein-1 can be used as one of the indicators for the diagnosis of spinal tuberculosis.
