CAJ | 학술논문

目的探讨高血压性脑出血患者血肿周围组织肿瘤坏死因子-α(tumor necrosis factor-α ,TNF-α)表达与血清TNF-α含量和血肿周围组织细胞凋亡的关系.方法 30例高血压性脑出血患者根据发病到手术时间随机分为<6 h组、6～12 h组、12～24 h组、24～48 h组、48～72 h组和>72 h组(每组n=5),各组患者临床基本情况、出血量、出血部位、术前格拉斯哥昏迷量表评分和手术持续时间均无显著差异.酶联免疫吸附双抗体夹心检测血清TNF-α含量,免疫组化法检测血肿周围组织TNF-α表达,TUNEL染色法检测血肿周围组织凋亡细胞.结果血肿周围组织TNF-α阳性细胞数量和血清TNF-α含量分别在出血后12～24 h和24～48 h达高峰,分别为(57.81±6.21)个/高倍视野和(78.45±7.17)pg/ml,且两者呈正相关(r=O.924,P<0.05);血肿周围组织TNF-α阳性细胞数量与凋亡细胞数量呈正相关(r=0.871,P<0.05).结论高血压性脑出血患者血清TNF-α含量显著增高,血肿周围组织TNF-α阳性细胞数量和凋亡细胞数量均显著增加.血清TNF-α含量与血肿周围组织TNF-α阳性细胞和凋亡细胞数量呈正相关.
목적 탐토고혈압성뇌출혈환자혈종주위조직종류배사인자-α(tumor necrosis factor-α ,TNF-α)표체여혈청TNF-α함량화혈종주위조직세포조망적관계.방법 30례고혈압성뇌출혈환자근거발병도수술시간수궤분위<6 h조、6～12 h조、12～24 h조、24～48 h조、48～72 h조화>72 h조(매조n=5),각조환자림상기본정황、출혈량、출혈부위、술전격랍사가혼미량표평분화수술지속시간균무현저차이.매련면역흡부쌍항체협심검측혈청TNF-α함량,면역조화법검측혈종주위조직TNF-α표체,TUNEL염색법검측혈종주위조직조망세포.결과 혈종주위조직TNF-α양성세포수량화혈청TNF-α함량분별재출혈후12～24 h화24～48 h체고봉,분별위(57.81±6.21)개/고배시야화(78.45±7.17)pg/ml,차량자정정상관(r=O.924,P<0.05);혈종주위조직TNF-α양성세포수량여조망세포수량정정상관(r=0.871,P<0.05).결론 고혈압성뇌출혈환자혈청TNF-α함량현저증고,혈종주위조직TNF-α양성세포수량화조망세포수량균현저증가.혈청TNF-α함량여혈종주위조직TNF-α양성세포화조망세포수량정정상관.
Objective To investigate the relationship between the expression of tumor necrosis factor-a (TNF-α) and the content of serum TNF-α in perihematomal tissue and apoptosis in patients with hypertensive intracerebral hemorrhage. Methods Thirty patients with hypertensive intracerebral hemorrhage were randomly divided into <6 h, 6-12 h, 12-24 h, 24-48 h, 48-72 h and >72 h groups according to the time from symptom onset to surgery (n =5 in each group). There were no significant differences among the basic clinical situation, amount of bleeding, bleeding sites, preoperative Glasgow coma scale scores and operation duration in all groups. A double-antibody sandwich enzyme-linked immunosorbent assay was used to determine the content of serum TNF-α in perihematomal tissue. Immunohistochemistry was used to detect the expression of TNF-α in perihematomal tissue, and TUNEL staining was used to detect the apoptotic cells. Results The numbers of TNF-α positive cells and the content of serum TNF-α in perihematomal tissue reached the peaks at 12-24 h and 24-48 h respectively after intracerebral hemorrhage. There was a positive correlation between them (r =0. 924, P <0. 05). The numbers of TNF-α positive cells in perihematomal tissue and the numbers of apoptotic cells were positively correlated (r =0.871, P <0. 05). Conclusions The content of serum TNF-α, the numbers of TNF-α positive cells and apoptotic cells in perihematomal tissue were increased significantly. The numbers of TNF-α positive cells in perihematomal tissue were positive correlated with the content of serum TNF-α and the numbers of apoptotic cells in perihematomal tissue.