CAJ | 학술논문

　　目的探讨含锶钙磷（SrCaP）涂层镁合金ZK60对小鼠前成骨细胞黏附、增殖和成骨分化等功能的影响。方法制备含SrCaP涂层的镁合金ZK60材料，以不含SrCaP涂层的ZK60和钛合金材料作为对照，扫描电镜观察材料表面的超微结构；以小鼠MC3T3-E1细胞为对象，扫描电镜、荧光显微镜、MTT法、p-NPP法等观察上述材料对细胞黏附、增殖和成骨分化的影响。结果扫描电镜可见含SrCaP涂层镁合金ZK60表面的粗糙多孔结构。与ZK60组相比，前成骨细胞在该材料上铺展面积更大，黏附更好；死活细胞染色显示培养24 h时SrCaP-ZK60组活细胞数最多；浸提液培养5 d时SrCaP涂层镁合金组细胞增殖显著高于钛合金组（P＜0.05）；培养7 d后，SrCaP涂层镁合金表面成骨细胞碱性磷酸酶表达显著高于ZK60组及钛合金组（P＜0.05）。结论含SrCaP涂层镁合金ZK60具有良好的促成骨细胞黏附、增殖和成骨分化作用。
　　목적탐토함송개린（SrCaP）도층미합금ZK60대소서전성골세포점부、증식화성골분화등공능적영향。방법제비함SrCaP도층적미합금ZK60재료，이불함SrCaP도층적ZK60화태합금재료작위대조，소묘전경관찰재료표면적초미결구；이소서MC3T3-E1세포위대상，소묘전경、형광현미경、MTT법、p-NPP법등관찰상술재료대세포점부、증식화성골분화적영향。결과소묘전경가견함SrCaP도층미합금ZK60표면적조조다공결구。여ZK60조상비，전성골세포재해재료상포전면적경대，점부경호；사활세포염색현시배양24 h시SrCaP-ZK60조활세포수최다；침제액배양5 d시SrCaP도층미합금조세포증식현저고우태합금조（P＜0.05）；배양7 d후，SrCaP도층미합금표면성골세포감성린산매표체현저고우ZK60조급태합금조（P＜0.05）。결론함SrCaP도층미합금ZK60구유량호적촉성골세포점부、증식화성골분화작용。
Objective To study the effects of SrCaP-coated Mg alloy ZK60 on murine preosteoblast functions of adherence, proliferation, and osteogenic differentiation in vitro. Methods SrCaP-coated Mg alloy ZK60 were prepared as the experiment goup, ZK60 and Titanium alloy were used as the control groups. The features of SrCaP-coated Mg alloy ZK60 surface were examined using scanning electron microscope (SEM). The effects of materials on adherence, proliferation and differentiation of murine MC3T3-E1 cells were observed by SEM, fluorescence microscope, MTT test and p-NPP assay. Results The results of SEM showed that a rough and porous surface morphology was formed on the coating of ZK60 alloy, and more adherent cells and larger spreading areas were observed on the surfaces of SrCaP-Coated Mg Alloy ZK60 than that on control groups. Maximum nomber of viable cells on SrCaP-Coated Mg Alloy ZK60 group at 24 h after culture were found by live/dead cell staining. MTT test showed that cell proliferation in SrCaP-coated Mg alloy group after 5 days' culture with alloy extracts was significantly higher than that in titanium alloy group (P <0.05), 7 d later, p-NPP activity assay demonstrated that alkaline phosphatase (ALP) level of the MC3T3-E1 in SrCaP-coated Mg alloy group was much higher than that in control groups (P <0.05). Conclusion The SrCaP-coated Mg alloy ZK60 could improve the early adherence, proliferation, and osteogenic differentiation of murine preosteoblast in vitro.