中国骨科临床与基础研究杂志
中國骨科臨床與基礎研究雜誌
중국골과림상여기출연구잡지
CHINESE JOURNAL OF CLINICAL AND BASIC ORTHO[AEDIC RESEARCH
2013年
1期
28-34
,共7页
钛%合金%晶粒细化%表面机械研磨%成骨细胞%骨涎蛋白%骨粘连蛋白%基因表达
鈦%閤金%晶粒細化%錶麵機械研磨%成骨細胞%骨涎蛋白%骨粘連蛋白%基因錶達
태%합금%정립세화%표면궤계연마%성골세포%골연단백%골점련단백%기인표체
Titanium%Alloys%Grain refinement%Surface mechanical attrition treatment%Osteoblasts%Bone sialoprotein%Osteonectin%Gene expressions
目的研究Ti-25Nb-3Mo-3Zr-2Sn（TLM）合金表面晶粒细化对HFOB1.19成骨细胞生物学行为的影响。方法实验分为两组，实验组（SMATed组）采用表面机械研磨处理（SMAT）方法在TLM合金表面制备一层β-Ti的纳米结构层，对照组（unSMATed组）采用未经SMAT的钛合金，将两组钛合金样品机械抛光至镜面。原子力显微镜分析两组样品表面的粗糙度及拓扑结构，光学显微镜及透射电镜分析表层晶粒大小，扫描电镜及MTT法分别考察成骨细胞的形态及细胞活力，并采用Real-time PCR技术分析材料对骨涎蛋白（BSP）及骨粘连蛋白（ON）基因表达的影响。结果抛光处理后的unSMATed组和SMATed组钛合金样品表面具有相近的微观粗糙度及拓扑结构，最表层的晶粒尺度分别为90±20μm及30±7 nm。成骨细胞与两组样品直接接触培养1、5、24、72、168 h，SMATed组样品表面细胞活性在各时间点均明显高于UnSMATed组（P＜0.05）；成骨细胞在两组样品表面培养3、7、14 d后，SMATed组样品表面BSP及ON的表达水平在各时相点均显著高于unSMATed组（P＜0.05）。结论TLM合金表面晶粒细化能显著促进成骨细胞的黏附、增殖及胞内特异性蛋白基因的表达，改善合金生物相容性。
目的研究Ti-25Nb-3Mo-3Zr-2Sn（TLM）閤金錶麵晶粒細化對HFOB1.19成骨細胞生物學行為的影響。方法實驗分為兩組，實驗組（SMATed組）採用錶麵機械研磨處理（SMAT）方法在TLM閤金錶麵製備一層β-Ti的納米結構層，對照組（unSMATed組）採用未經SMAT的鈦閤金，將兩組鈦閤金樣品機械拋光至鏡麵。原子力顯微鏡分析兩組樣品錶麵的粗糙度及拓撲結構，光學顯微鏡及透射電鏡分析錶層晶粒大小，掃描電鏡及MTT法分彆攷察成骨細胞的形態及細胞活力，併採用Real-time PCR技術分析材料對骨涎蛋白（BSP）及骨粘連蛋白（ON）基因錶達的影響。結果拋光處理後的unSMATed組和SMATed組鈦閤金樣品錶麵具有相近的微觀粗糙度及拓撲結構，最錶層的晶粒呎度分彆為90±20μm及30±7 nm。成骨細胞與兩組樣品直接接觸培養1、5、24、72、168 h，SMATed組樣品錶麵細胞活性在各時間點均明顯高于UnSMATed組（P＜0.05）；成骨細胞在兩組樣品錶麵培養3、7、14 d後，SMATed組樣品錶麵BSP及ON的錶達水平在各時相點均顯著高于unSMATed組（P＜0.05）。結論TLM閤金錶麵晶粒細化能顯著促進成骨細胞的黏附、增殖及胞內特異性蛋白基因的錶達，改善閤金生物相容性。
목적연구Ti-25Nb-3Mo-3Zr-2Sn（TLM）합금표면정립세화대HFOB1.19성골세포생물학행위적영향。방법실험분위량조，실험조（SMATed조）채용표면궤계연마처리（SMAT）방법재TLM합금표면제비일층β-Ti적납미결구층，대조조（unSMATed조）채용미경SMAT적태합금，장량조태합금양품궤계포광지경면。원자력현미경분석량조양품표면적조조도급탁복결구，광학현미경급투사전경분석표층정립대소，소묘전경급MTT법분별고찰성골세포적형태급세포활력，병채용Real-time PCR기술분석재료대골연단백（BSP）급골점련단백（ON）기인표체적영향。결과포광처리후적unSMATed조화SMATed조태합금양품표면구유상근적미관조조도급탁복결구，최표층적정립척도분별위90±20μm급30±7 nm。성골세포여량조양품직접접촉배양1、5、24、72、168 h，SMATed조양품표면세포활성재각시간점균명현고우UnSMATed조（P＜0.05）；성골세포재량조양품표면배양3、7、14 d후，SMATed조양품표면BSP급ON적표체수평재각시상점균현저고우unSMATed조（P＜0.05）。결론TLM합금표면정립세화능현저촉진성골세포적점부、증식급포내특이성단백기인적표체，개선합금생물상용성。
Objective To investigate the effect of surface grain refinement of Ti-25Nb-3Mo-3Zr-2Sn (TLM) alloy on the regulation of HFOB1.19 osteoblast behavior. Methods The experiment was designed as two groups:nanocrystalline layer with pure β-Ti was fabricated by surface mechanical attrition treatment (SMAT) on TLM alloy in experimental group (SMATed group), and the alloy in control group were not be treated by SMAT (unSMAT group). Subsequently, the SMATed and unSMATed samples were mechanically polished to mirror finish. Surface roughness and topography of the samples in two groups were analyzed by atomic force microscope, grain sizes in the surface layer were observed by optical microscopy and transmission electron microscopy, obsteoblast morphology and viability were examined by scanning electron microscope and MTT method respectively, and gene expressions of bone sialoprotein (BSP) and osteonectin (ON) were evaluated by Real-time PCR. Results The samples in two groups showed similar surface roughness and topography, and the grain size in the exposed surface layers of unSMATed and SMATed samples was 90 ± 20 μm and 30 ± 7 nm, respectively. At 1, 5, 24, 72, 168 h after the direct culture of obsteoblast and the samples, cell viability on the surface of SMATed samples were better than that of unSMATed samples (P <0.05); At 3, 7, 14 d after the culture of obsteoblast and the samples, BSP and ON gene expressions on the surface of SMATed samples were higher than those of unSMATed samples (P <0.05). Conclusions For TLM alloy, surface grain refinement can significantly promote osteoblast adhesion, proliferation, intracellular specific protein-related gene expressions, and improve its cell biocompatibility.
