CAJ | 학술논문

　　目的制备具备关节解剖形态的组织工程骨软骨，行原位移植修复兔关节大面积缺损，评价修复后效果。方法制备具有解剖形态的明胶－硫酸软骨素－透明质酸钠－胶原－陶瓷化骨软骨支架。分为复合细胞组，无细胞组和不修复组。进行体内原位移植，术后不同时间进行大体观、运动功能检查、HE染色观察，并进行O’Driscoll评分。结果复合细胞组与其他两组相比，运动功能基本无障碍，前行时双下肢运动对称，大体观可见与正常关节外形接近，表面软骨层呈现略透明白色，与正常组织界限不明显，HE染色可见组织结构逐步改建与正常关节组织结构类似。结论复合组织在功能恢复中起到了相当大的作用，但与正常关节还有较大差距。
　　목적제비구비관절해부형태적조직공정골연골，행원위이식수복토관절대면적결손，평개수복후효과。방법제비구유해부형태적명효－류산연골소－투명질산납－효원－도자화골연골지가。분위복합세포조，무세포조화불수복조。진행체내원위이식，술후불동시간진행대체관、운동공능검사、HE염색관찰，병진행O’Driscoll평분。결과복합세포조여기타량조상비，운동공능기본무장애，전행시쌍하지운동대칭，대체관가견여정상관절외형접근，표면연골층정현략투명백색，여정상조직계한불명현，HE염색가견조직결구축보개건여정상관절조직결구유사。결론복합조직재공능회복중기도료상당대적작용，단여정상관절환유교대차거。
Objective We aimed to discuss the results of anatomical osteochondral composites in repairing large articular joint defects. Methods We prepared gelatin-chondroitin sulfate-hyaluronic acid and gelatin-ceramic anatomical shape osteochondral composites scaffold, and divided the patients into composite cell group, no cell group and not repair group. In situ transplantation in the body, observe the general view, movement function, HE dyeing, and O'Driscoll score after surgery in the different time. Results In experiment group there was obstacle in movement function. When they were walking, lower limb movement was symmetrical, general view was as normal as nature joint, surface layer presented slightly hyaline cartilage, and normal tissue boundary was not obvious. HE stain showed organization structure’s gradual renovation and its similarity to natural joints. Conclusion Anatomical composite tissue played a key role in function recovery through the postoperative movement function, and general observation. However, it still needed further deepening research.