漳州师范学院学报（自然科学版）
漳州師範學院學報（自然科學版）
장주사범학원학보（자연과학판）
JOURNAL OF ZHANGZHOU TEACHERS COLLEGE(NATURAL SCIENCE EDITION)
2013年
2期
65-69
,共5页
蔡文燕%翁秀兰%王江森%温秀萍%陈泉清
蔡文燕%翁秀蘭%王江森%溫秀萍%陳泉清
채문연%옹수란%왕강삼%온수평%진천청
千层金%叶片%愈伤组织%诱导%分化
韆層金%葉片%愈傷組織%誘導%分化
천층금%협편%유상조직%유도%분화
Melaleuca bracteata F. Muell.%leaves%callus%induction%differentiation
以千层金（Melaleuca bracteata F. Muell.）的幼嫩叶片为外植体,探讨不同灭菌方法对外植体的表面灭菌效果及不同植物生长调节剂组合与浓度配比对千层金叶片愈伤组织诱导及分化的影响.结果表明：75%酒精浸泡30s+1%次氯酸钠溶液灭菌15min 的组合为千层金叶片外植体适宜的灭菌方法；千层金叶片在附加2,4-D、NAA 和6-BA 的 MS 培养基上均能诱导出愈伤组织,其中诱导愈伤组织的最佳培养基为MS+NAA2mg/L+6-BA0.1mg/L，诱导率可达100%，诱导不定芽分化的最佳培养基为 MS+6-BA1mg/L+NAA0.1mg/L，分化率为19.8%.
以韆層金（Melaleuca bracteata F. Muell.）的幼嫩葉片為外植體,探討不同滅菌方法對外植體的錶麵滅菌效果及不同植物生長調節劑組閤與濃度配比對韆層金葉片愈傷組織誘導及分化的影響.結果錶明：75%酒精浸泡30s+1%次氯痠鈉溶液滅菌15min 的組閤為韆層金葉片外植體適宜的滅菌方法；韆層金葉片在附加2,4-D、NAA 和6-BA 的 MS 培養基上均能誘導齣愈傷組織,其中誘導愈傷組織的最佳培養基為MS+NAA2mg/L+6-BA0.1mg/L，誘導率可達100%，誘導不定芽分化的最佳培養基為 MS+6-BA1mg/L+NAA0.1mg/L，分化率為19.8%.
이천층금（Melaleuca bracteata F. Muell.）적유눈협편위외식체,탐토불동멸균방법대외식체적표면멸균효과급불동식물생장조절제조합여농도배비대천층금협편유상조직유도급분화적영향.결과표명：75%주정침포30s+1%차록산납용액멸균15min 적조합위천층금협편외식체괄의적멸균방법；천층금협편재부가2,4-D、NAA 화6-BA 적 MS 배양기상균능유도출유상조직,기중유도유상조직적최가배양기위MS+NAA2mg/L+6-BA0.1mg/L，유도솔가체100%，유도불정아분화적최가배양기위 MS+6-BA1mg/L+NAA0.1mg/L，분화솔위19.8%.
Using the tender leaves of Melaleuca bracteata F. Muell. as explants, the effect of different disinfection methods on explants and the influence of plant growth regulators on callus induction and differentiation were studied. The results showed that the disinfection method of 75% alcohol to deal with for 30 s and 1% NaClO to deal with for 15 min was suitable for tender leaves. Callus could be induced on MS medium supplemented with different combinations of 2,4-D,NAA and 6-BA. The optimum medium for callus induction was MS+ NAA2mg/L +6-BA 0.1mg/L with 100% of callus induction rate. The optimum medium for bud differentiation of callus was MS+6-BA 1mg/L +NAA0.1mg/L with 19.8% of differentiation rate.
