CAJ | 학술논문

　　目的克隆特异AT序列结合蛋白1（special AT rich sequence binding protein，SATB1）基因全长编码序列，构建及鉴定其真核表达载体，为研究SATB1的功能及作用机制奠定实验基础。方法以cDNA文库提供的pCMV6-XL6-SATB1为模板，通过PCR扩增SATB1基因全长编码序列，克隆入pGEM-T载体，再将SATB1基因插入真核表达载体pEGFP-N1，构建重组真核表达载体pEGFP-SATB1。结果克隆的SATB1基因编码序列全长2312 bp，经测序比对与Genbank登记的序列(BC001744.1)完全一致。经酶切及测序证实，SATB1基因正确插入pEGFP-N1载体中。结论 SATB1基因全长编码序列克隆正确，重组真核表达载体pEGFP-SATB1构建成功。
　　목적극륭특이AT서렬결합단백1（special AT rich sequence binding protein，SATB1）기인전장편마서렬，구건급감정기진핵표체재체，위연구SATB1적공능급작용궤제전정실험기출。방법이cDNA문고제공적pCMV6-XL6-SATB1위모판，통과PCR확증SATB1기인전장편마서렬，극륭입pGEM-T재체，재장SATB1기인삽입진핵표체재체pEGFP-N1，구건중조진핵표체재체pEGFP-SATB1。결과극륭적SATB1기인편마서렬전장2312 bp，경측서비대여Genbank등기적서렬(BC001744.1)완전일치。경매절급측서증실，SATB1기인정학삽입pEGFP-N1재체중。결론 SATB1기인전장편마서렬극륭정학，중조진핵표체재체pEGFP-SATB1구건성공。
Objective To clone the cDNA of the gene encoding special AT-rich sequence binding protein(SATB1)and to subclone it into eukaryotic expression vector pEGFP in order to investigate the function and mechanism of action of SATB1. Methods SATB1 cDNA was cloned from pCMV6-XL6-SATB1 by polymerase chain reaction(PCR)and then the target gene was subcloned into pGEM-T and subsequently into the eukaryotic expression vector pEGFP-N1 to give pEGFP-SATB1. Results The complete cDNA sequence of SATB1(2312 bp)was consistent with the reported sequence(Genbank BC001744.1).Sequencing and restriction digest analysis re-vealed the target gene to be inserted in the expression vector in the correct orientation. Conclusion The SATB1 cDNA was success-fully cloned and used to construct recombinant eukaryotic expression vector pEGFP-SATB1.