CAJ | 학술논문

　　目的检测坐落于甲胎蛋白（α-fetoprotein，AFP）基因候选单核苷酸多态性（single nucleotide polymorphisms，SNPs）位点rs 12506899、rs 16849384和位于白蛋白（albumin，ALB）基因候选SNP位点rs 7688274基因型在肝癌细胞株中的分布及其对肝癌细胞株AFP水平的影响。方法选取AFP表达阴性的永生化正常肝细胞株HL-7702及AFP表达阳性的肝癌细胞株BEL-7402、BEL-7404、Hep-G2、MHCC-97H、SMMC-7721进行培养，检测其培养液中AFP的水平，提取细胞DNA，以Taq-man MGB探针法进行基因分型，对各组基因型间AFP的水平进行比较。结果 rs 16849384 T/T基因型的Hep-G2的AFP水平显著高于C/T基因型的SMMC-7721、HL-7702和BEL-7402（P分别为<0.001、0.001和0.015）。结论[1]坐落于AFP基因的rs 16849384基因型与肝癌细胞株的AFP水平相关；[2]在肝癌细胞株中，位点rs 16849384等位基因T为高值等位基因。
　　목적검측좌락우갑태단백（α-fetoprotein，AFP）기인후선단핵감산다태성（single nucleotide polymorphisms，SNPs）위점rs 12506899、rs 16849384화위우백단백（albumin，ALB）기인후선SNP위점rs 7688274기인형재간암세포주중적분포급기대간암세포주AFP수평적영향。방법선취AFP표체음성적영생화정상간세포주HL-7702급AFP표체양성적간암세포주BEL-7402、BEL-7404、Hep-G2、MHCC-97H、SMMC-7721진행배양，검측기배양액중AFP적수평，제취세포DNA，이Taq-man MGB탐침법진행기인분형，대각조기인형간AFP적수평진행비교。결과 rs 16849384 T/T기인형적Hep-G2적AFP수평현저고우C/T기인형적SMMC-7721、HL-7702화BEL-7402（P분별위<0.001、0.001화0.015）。결론[1]좌락우AFP기인적rs 16849384기인형여간암세포주적AFP수평상관；[2]재간암세포주중，위점rs 16849384등위기인T위고치등위기인。
Objective To explore the influences of the rs 12506899 and rs 16849384 polymorphisms in theα-fetoprotein(AFP)gene and the rs 7688274 polymorphism in the albumin(ALB)gene on AFP levels in human hepatoma cell lines. Methods AFP levels in the medium were measured for cultures of AFP-negative normal human cell line HL-7702 and AFP-positive human hepatoma cell lines BEL-7402,BEL-7404,Hep-G2,MHCC-97H and SMMC-7721.DNA was extracted and genotyped by real-time PCR(Taq-man assay). AFP levels were compared for hepatoma cell lines with different genotypes. Results A statistically significant association was observed between rs 16849384 and AFP levels.AFP levels were higher in Hep-G2 cells carrying a T/T genotype at rs 16849384 than in SMMC-7721,HL-7702 and BEL-7402 cells carrying a C/T genotype(P<0.001,<0.001 and<0.015,respectively). Conclusions Genotypes of rs 16849384 may be associated with AFP levels in hepatoma cell lines,and the T allele of rs 16849384 is associated with higher AFP level.