CAJ | 학술논문

　　目的建立稳定敲除GLRX3（glutaredoxin 3）基因的鼻咽癌细胞株，探索GLRX3基因在鼻咽癌细胞中的生物学功能。方法构建人GLRX3基因的shRNA逆转录病毒载体，将其转染入鼻咽癌细胞株HONE1中，筛选、建立稳定敲除GLRX3基因的HONE1细胞株。采用实时荧光定量PCR法验证筛选细胞中GLRX3基因的转录水平，通过克隆形成实验和划痕实验检测敲除GLRX3基因后鼻咽癌细胞克隆形成能力及迁移运动能力，评价敲除GLRX3基因后鼻咽癌细胞生物学行为的改变情况。结果成功构建的4个GLRX3 shRNA-pBINNS2质粒载体均可抑制GLRX3基因的转录表达。敲除GLRX3基因的HONE1细胞的克隆形成能力及迁移运动能力均降低。结论敲除GLRX3基因可抑制鼻咽癌细胞的恶性生物学行为，GLRX3可能是鼻咽癌候选的癌基因。
　　목적건립은정고제GLRX3（glutaredoxin 3）기인적비인암세포주，탐색GLRX3기인재비인암세포중적생물학공능。방법구건인GLRX3기인적shRNA역전록병독재체，장기전염입비인암세포주HONE1중，사선、건립은정고제GLRX3기인적HONE1세포주。채용실시형광정량PCR법험증사선세포중GLRX3기인적전록수평，통과극륭형성실험화화흔실험검측고제GLRX3기인후비인암세포극륭형성능력급천이운동능력，평개고제GLRX3기인후비인암세포생물학행위적개변정황。결과성공구건적4개GLRX3 shRNA-pBINNS2질립재체균가억제GLRX3기인적전록표체。고제GLRX3기인적HONE1세포적극륭형성능력급천이운동능력균강저。결론고제GLRX3기인가억제비인암세포적악성생물학행위，GLRX3가능시비인암후선적암기인。
Objective To establish glutaredoxin 3(GLRX3)-knocked down nasopharyngeal carcinoma(NPC)cell lines and explore the biological functions of the GLRX3 gene. Methods Retroviral vectors expressing shRNA against GLRX3 were constructed and transfected into HONE1 cells,and stable cell lines showing knocked-down GLRX3 expression were selected. Reduced GLRX3 tran-scription in the cell lines was confirmed by real-time quantitative PCR.The cells were examined in colony formation and wound heal-ing assays to explore the influence of GLRX3 on these processes. Results Four retroviral shRNA vectors targeting GLRX3(shRNA-pBINNS2)were constructed and stably transfected into HONE1 cells.GLRX3 transcription was effectively suppressed in the stable cell lines.Knocking down GLRX3 inhibited clonogenicity and migration. Conclusions Knocking down GLRX3 inhibits the malignant behavior of NPC cells,suggesting that GLRX3 is a candidate oncogene in NPC.