CAJ | 학술논문

　　从杭州植物园土样中筛选到1株高产果胶酶菌株，经鉴定为黑曲霉，命名为黑曲霉JL15（As-pergillus niger JL15）。固体发酵条件研究结果表明，以麸皮为主要基质，用橘皮粉替代10%质量的麸皮可提高果胶酶的产量，其外加碳源、氮源分别以甘油和硫酸铵最优，最适含水量60 g/100g，最适培养时间72 h~84 h。黑曲霉果胶酶的Km为4.04 mg/mL， vmax为40.16μmol/min·mg。
　　종항주식물완토양중사선도1주고산과효매균주，경감정위흑곡매，명명위흑곡매JL15（As-pergillus niger JL15）。고체발효조건연구결과표명，이부피위주요기질，용귤피분체대10%질량적부피가제고과효매적산량，기외가탄원、담원분별이감유화류산안최우，최괄함수량60 g/100g，최괄배양시간72 h~84 h。흑곡매과효매적Km위4.04 mg/mL， vmax위40.16μmol/min·mg。
A fungi strains which produces pectinase was isolated from the Hangzhou Botanical garden soil and named Aspergillus niger JL 15. The conditions of pectinase production from Aspergillus niger JL15 in solid state fermentation (SSF) and its hydrolytic properties were studied. The results showed that the best adding carbon and nitrogen sources for pectinase production were glycerol and ammonium sulfate,respectively. The pectinase production could be improved by substituting wheat bran with 10% orange peel powder. The best initial moisture was 60 g/100g . The optimal incubation time was 72 h~84 h. The pectinase exhibited Km and vmax values were 4.04 mg/mL and 40.16μmol/min·mg, respectively.