CAJ | 학술논문

　　目的观察银杏叶提取物（extracts of Ginkgo biloba leaf, EGB）对大鼠脑缺血再灌注氧化损伤的保护作用，为进一步开展复方候选新药筛选研究提供参考依据。方法雄性Wistar大鼠60只，随机等分为①对照组，②模型组，③假手术组，④EGB低剂量组，⑤EGB中剂量组，⑥EGB高剂量组。第①～③组灌胃给予蒸馏水l0 ml/kg，第④～⑥组分别灌胃给予含EGB 25、50、100 mg/kg的蒸馏水混悬液l0 ml/kg，连续14 d。最后一次灌胃后1h，②、④、⑤、⑥组大鼠均结扎双颈总动脉造成不完全性脑缺血1 h，复灌30 min；①组不做处理，③组仅做手术处理不结扎颈总动脉。取各组大鼠大脑皮层制备组织匀浆，检测超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)活性，谷胱甘肽(GSH)和丙二醛(MDA)含量以及总抗氧化能力(T-AOC)等指标的变化。结果与对照组相比，模型组脑组织中的SOD活性、GSH含量和T-AOC显著下降，而NOS活性和MDA含量明显升高；与模型组相比，给于EGB使脑组织中SOD活性、GSH含量和T-AOC明显升高，NOS活性和MDA含量明显降低，中剂量的效果较好。结论 EGB对大鼠脑缺血再灌注氧化损伤具有明显保护作用，且呈一定的剂量相关特性。
　　목적관찰은행협제취물（extracts of Ginkgo biloba leaf, EGB）대대서뇌결혈재관주양화손상적보호작용，위진일보개전복방후선신약사선연구제공삼고의거。방법웅성Wistar대서60지，수궤등분위①대조조，②모형조，③가수술조，④EGB저제량조，⑤EGB중제량조，⑥EGB고제량조。제①～③조관위급여증류수l0 ml/kg，제④～⑥조분별관위급여함EGB 25、50、100 mg/kg적증류수혼현액l0 ml/kg，련속14 d。최후일차관위후1h，②、④、⑤、⑥조대서균결찰쌍경총동맥조성불완전성뇌결혈1 h，복관30 min；①조불주처리，③조부주수술처리불결찰경총동맥。취각조대서대뇌피층제비조직균장，검측초양화물기화매(SOD)화일양화담합매(NOS)활성，곡광감태(GSH)화병이철(MDA)함량이급총항양화능력(T-AOC)등지표적변화。결과여대조조상비，모형조뇌조직중적SOD활성、GSH함량화T-AOC현저하강，이NOS활성화MDA함량명현승고；여모형조상비，급우EGB사뇌조직중SOD활성、GSH함량화T-AOC명현승고，NOS활성화MDA함량명현강저，중제량적효과교호。결론 EGB대대서뇌결혈재관주양화손상구유명현보호작용，차정일정적제량상관특성。
Objective We tried to observe the protective effects of extracts of Ginkgo biloba leaf(EGB) on cerebral ischemia/reperfusion induced oxidative injury in rats. Methods Sixty male Wistar rats were randomly divided into following six groups(n=10for each): (a)normal control,(b) injury model,(c)sham operation,(d)low dose of EGB,(e)middle dose of EGB,(f) high dose of EGB. EGB dissolved in distilled in water at dosages of 25,50 and 100mg/kg was given to group d,e,f by gastric tube, continued for 14d; but group a,b,c was only given the equal volume of distilled water at dose of 10ml/kg. At 1 h after the last drug administration, the rats in group b,d,e,f were submitted to incomplete cerebral ischemia(1h)/reperfusion(30min) injury;but group c was only operated and not submitted to cerebral ischemia/reperfusion injury. The cerebral cortex tissue was taken and homogenized for following index assays:SOD and NOS activities, GSH,MDA content and total antioxidative catacity(T-AOC). Results Compared with the normal control, the SOD activity, GSH content and T-AOC in rat brain significantly decreased, but NOS activity and MDA content significantly increased in injury model group. Compared with the injury model, SOD activity, GSH content and T-AOC in rat brain significantly increased, but NOS activity and MDA content were significantly decreased after administration of EGB,especially at dosage of 50 mg/kg. Conclusions EGB has protective effects on cerebral ischemia/reperfusion induced oxidative injury in rats and shared the characteristic of dose-related effect.