湖南师范大学学报(医学版)
湖南師範大學學報(醫學版)
호남사범대학학보(의학판)
JOURNAL OF HUNAN NORMAL UNIVERSITY(MEDICAL SCIENCE)
2013年
2期
49-52
,共4页
袁鲲%邬靖敏%豆清娅%李军%邹明祥%Zou Mingxiang
袁鯤%鄔靖敏%豆清婭%李軍%鄒明祥%Zou Mingxiang
원곤%오정민%두청아%리군%추명상%Zou Mingxiang
无菌部位%金黄色葡萄球菌%耐药性%分子流行病学
無菌部位%金黃色葡萄毬菌%耐藥性%分子流行病學
무균부위%금황색포도구균%내약성%분자류행병학
sterile sites%staphylococcus aureus%antibiotics resistance%molecular epidemiology
目的:了解无菌部位分离的金黄色葡萄球菌对常用抗菌药物的耐药性及分子流行病学特征,为临床预防和控制其感染以及合理使用抗菌药物提供实验依据。方法:收集2011年8月至2012年6月某三甲医院无菌部位分离的非重复金黄色葡萄球菌50株。 VITEK-2微生物分析系统进行菌株鉴定,PCR检测耐甲氧西林的金黄色葡萄球菌(MRSA)杀白细胞毒素(PVL)基因,多重PCR对MRSA进行SCCmec基因分型。结果:在18种抗菌药物中,青霉素和红霉素的耐药率最高,分别为90.0%和62.0%;尚无对万古霉素、替加环素、替考拉宁、利奈唑胺、达托霉素的耐药菌株。50株金葡菌中,MRSA 15株(30.0%),MSSA35株(70.0%),MRSA对12种抗菌药物的敏感率明显低于MSSA。15株MRSA中,SCCmecⅢ型11株(73.3%),SCCmecⅣ型4株(26.7%),PVL基因扩增均为阴性。结论:该院无菌部位分离的金黄色葡萄球菌以MSSA为主;MRSA对抗菌药物呈多重耐药性,且以SCCmecⅢ型为主。
目的:瞭解無菌部位分離的金黃色葡萄毬菌對常用抗菌藥物的耐藥性及分子流行病學特徵,為臨床預防和控製其感染以及閤理使用抗菌藥物提供實驗依據。方法:收集2011年8月至2012年6月某三甲醫院無菌部位分離的非重複金黃色葡萄毬菌50株。 VITEK-2微生物分析繫統進行菌株鑒定,PCR檢測耐甲氧西林的金黃色葡萄毬菌(MRSA)殺白細胞毒素(PVL)基因,多重PCR對MRSA進行SCCmec基因分型。結果:在18種抗菌藥物中,青黴素和紅黴素的耐藥率最高,分彆為90.0%和62.0%;尚無對萬古黴素、替加環素、替攷拉寧、利奈唑胺、達託黴素的耐藥菌株。50株金葡菌中,MRSA 15株(30.0%),MSSA35株(70.0%),MRSA對12種抗菌藥物的敏感率明顯低于MSSA。15株MRSA中,SCCmecⅢ型11株(73.3%),SCCmecⅣ型4株(26.7%),PVL基因擴增均為陰性。結論:該院無菌部位分離的金黃色葡萄毬菌以MSSA為主;MRSA對抗菌藥物呈多重耐藥性,且以SCCmecⅢ型為主。
목적:료해무균부위분리적금황색포도구균대상용항균약물적내약성급분자류행병학특정,위림상예방화공제기감염이급합리사용항균약물제공실험의거。방법:수집2011년8월지2012년6월모삼갑의원무균부위분리적비중복금황색포도구균50주。 VITEK-2미생물분석계통진행균주감정,PCR검측내갑양서림적금황색포도구균(MRSA)살백세포독소(PVL)기인,다중PCR대MRSA진행SCCmec기인분형。결과:재18충항균약물중,청매소화홍매소적내약솔최고,분별위90.0%화62.0%;상무대만고매소、체가배소、체고랍저、리내서알、체탁매소적내약균주。50주금포균중,MRSA 15주(30.0%),MSSA35주(70.0%),MRSA대12충항균약물적민감솔명현저우MSSA。15주MRSA중,SCCmecⅢ형11주(73.3%),SCCmecⅣ형4주(26.7%),PVL기인확증균위음성。결론:해원무균부위분리적금황색포도구균이MSSA위주;MRSA대항균약물정다중내약성,차이SCCmecⅢ형위주。
Objective To understand antibiotic resistance and molecular epidemiology characteristics of Staphylococcus aureus isolated from sterile sites, thus provide evidence for prevention and control of Staphylococ-cus aureus infections, and for the rational use of antibiotics in clinical settings. Methods A total of 50 non-dupli-cate clinical isolates of Staphylococcus aureus from sterile sites were collected from August 2011 to June 2012 in a hospital and then identified by Vitek-2 microbiological analysis system.The Panton-Valentine leukocidin (PVL) gene was detected by simple PCR and the genotypes of staphylococcals chromosomal cassette mec(SCCmec) were determined by multiplex PCR. Results Among the 18 antibiotics, resistance rates to penicillin and erythromycin were the highest (90.0% and 62.0% respectively). No isolates resistant to vancomycin, tigecycline, teicoplanin, linezolid and daptomycin were found. Among the 50 Staphylococcus aureus strains, there were 15 strains of MR-SA(30.0%) and 35 strains of MSSA(70.0%). The sensitivities of MRSA to the other 12 antibiotics were much lower than that of MSSA. Among the 15 strains of MRSA, 11 (73.3%) strains belonged to SCCmec typeⅢ,4 (26.7%) strains belonged to SCCmec typeⅣ, PVL genes were all negative. Conclusions Staphylococcus aureus isolated from sterile sites were major MSSA, but MRSA presented with multi-drug resistance to antibiotics and a major genotype of SCCmec typeⅢ.