保鲜与加工
保鮮與加工
보선여가공
STORAGE PROCESS
2013年
4期
24-27
,共4页
蒽酮%比色法%甜玉米%可溶性糖%测定
蒽酮%比色法%甜玉米%可溶性糖%測定
은동%비색법%첨옥미%가용성당%측정
anthrone%colorimetric method%sweet corn%soluble sugar%determination
以甜玉米为试材，通过对蒽酮浓度、蒽酮-糠醛显色反应温度和时间的探讨，研究优化蒽酮比色法测定甜玉米中可溶性糖含量的反应条件。结果表明，蒽酮比色法适宜的测定条件为：蒽酮的最佳浓度15 mg/mL，显色反应温度80℃，加热时间15 min。在此条件下葡萄糖标准品浓度与吸光度之间具有良好的线性关系（y=15.954 x+0.0976，R2=0.9992）。采用此优化条件测定甜玉米籽粒中可溶性糖含量，其测定结果具有良好的重复性（RSD为0.47%）和准确度（平均回收率为101.92%）。
以甜玉米為試材，通過對蒽酮濃度、蒽酮-糠醛顯色反應溫度和時間的探討，研究優化蒽酮比色法測定甜玉米中可溶性糖含量的反應條件。結果錶明，蒽酮比色法適宜的測定條件為：蒽酮的最佳濃度15 mg/mL，顯色反應溫度80℃，加熱時間15 min。在此條件下葡萄糖標準品濃度與吸光度之間具有良好的線性關繫（y=15.954 x+0.0976，R2=0.9992）。採用此優化條件測定甜玉米籽粒中可溶性糖含量，其測定結果具有良好的重複性（RSD為0.47%）和準確度（平均迴收率為101.92%）。
이첨옥미위시재，통과대은동농도、은동-강철현색반응온도화시간적탐토，연구우화은동비색법측정첨옥미중가용성당함량적반응조건。결과표명，은동비색법괄의적측정조건위：은동적최가농도15 mg/mL，현색반응온도80℃，가열시간15 min。재차조건하포도당표준품농도여흡광도지간구유량호적선성관계（y=15.954 x+0.0976，R2=0.9992）。채용차우화조건측정첨옥미자립중가용성당함량，기측정결과구유량호적중복성（RSD위0.47%）화준학도（평균회수솔위101.92%）。
Taking sweet corn as test material, through the study of anthrone concentration, the temperature and time of color reaction of anthrone - furfuralx, the reaction conditions of the anthrone colorimetric method to determine the soluble sugar content of sweet corn were studied. The results showed that, the optimum conditions of anthrone colorimetric method were as follows:the optimum concentration of anthrone was 15 mg/mL, temperature of color reaction was 80℃, heating time was 15 min. Under these conditions, there was a good linear relationship between concentration of the glucose standard and absorbance (y=15.954 x+0.097 6, R2=0.999 2). With this optimized method, the determination results of the soluble sugar in sweet corn had good repeatability (RSD 0.47%) and accuracy (average recovery rate 101.92%).
