中国医药导刊
中國醫藥導刊
중국의약도간
CHINESE JOURNAL OF MEDICAL GUIDE
2013年
6期
1030-1032
,共3页
VEGF-A%VEGF-C%宫颈癌%ELISA
VEGF-A%VEGF-C%宮頸癌%ELISA
VEGF-A%VEGF-C%궁경암%ELISA
VEGF-A%VEGF-C%Cervical cancer%ELISA
目的：检测血管内皮生长因子A和C(VEGF-A、VEGF-C)在宫颈癌血清中的表达并探讨其在早期宫颈癌诊断价值，以期有助于宫颈癌生长和转移机制的进一步阐明。方法：采用ELISA法检测正常宫颈组10例，宫颈上皮内瘤变CINⅢ组36例，宫颈癌无淋巴结转移患者组33例，宫颈癌有淋巴结转移患者组10例，检测血清中VEGF-A、VEGF-C的浓度表达并比较各组间差异性。结果：(1)宫颈癌无淋巴结转移者组VEGF-A、VEGF-C血清水平均高于正常宫颈组及CINⅢ组，差异有统计学意义(P<0.05)；(2)宫颈癌有淋巴结转移者组VEGF-A、VEGF-C血清水平均高于宫颈癌无淋巴结转移组，差异有统计学意义(P<0.05)；(3)CINⅢ组VEGF-A、VEGF-C血清水平与正常宫颈组相比，差异无统计学无意义(P>0.05)。结论：宫颈癌患者血清VEGF-A、VEGF-C水平升高可能与宫颈癌淋巴结转移密切相关，可能成为推测宫颈癌淋巴结转移和预后的有用的生物学指标；其增高与淋巴结转移密切相关，提示其可能预示宫颈癌不良预后。另外，两者在宫颈癌组织中表达增高，可能是宫颈癌发生发展的原因之一。
目的：檢測血管內皮生長因子A和C(VEGF-A、VEGF-C)在宮頸癌血清中的錶達併探討其在早期宮頸癌診斷價值，以期有助于宮頸癌生長和轉移機製的進一步闡明。方法：採用ELISA法檢測正常宮頸組10例，宮頸上皮內瘤變CINⅢ組36例，宮頸癌無淋巴結轉移患者組33例，宮頸癌有淋巴結轉移患者組10例，檢測血清中VEGF-A、VEGF-C的濃度錶達併比較各組間差異性。結果：(1)宮頸癌無淋巴結轉移者組VEGF-A、VEGF-C血清水平均高于正常宮頸組及CINⅢ組，差異有統計學意義(P<0.05)；(2)宮頸癌有淋巴結轉移者組VEGF-A、VEGF-C血清水平均高于宮頸癌無淋巴結轉移組，差異有統計學意義(P<0.05)；(3)CINⅢ組VEGF-A、VEGF-C血清水平與正常宮頸組相比，差異無統計學無意義(P>0.05)。結論：宮頸癌患者血清VEGF-A、VEGF-C水平升高可能與宮頸癌淋巴結轉移密切相關，可能成為推測宮頸癌淋巴結轉移和預後的有用的生物學指標；其增高與淋巴結轉移密切相關，提示其可能預示宮頸癌不良預後。另外，兩者在宮頸癌組織中錶達增高，可能是宮頸癌髮生髮展的原因之一。
목적：검측혈관내피생장인자A화C(VEGF-A、VEGF-C)재궁경암혈청중적표체병탐토기재조기궁경암진단개치，이기유조우궁경암생장화전이궤제적진일보천명。방법：채용ELISA법검측정상궁경조10례，궁경상피내류변CINⅢ조36례，궁경암무림파결전이환자조33례，궁경암유림파결전이환자조10례，검측혈청중VEGF-A、VEGF-C적농도표체병비교각조간차이성。결과：(1)궁경암무림파결전이자조VEGF-A、VEGF-C혈청수평균고우정상궁경조급CINⅢ조，차이유통계학의의(P<0.05)；(2)궁경암유림파결전이자조VEGF-A、VEGF-C혈청수평균고우궁경암무림파결전이조，차이유통계학의의(P<0.05)；(3)CINⅢ조VEGF-A、VEGF-C혈청수평여정상궁경조상비，차이무통계학무의의(P>0.05)。결론：궁경암환자혈청VEGF-A、VEGF-C수평승고가능여궁경암림파결전이밀절상관，가능성위추측궁경암림파결전이화예후적유용적생물학지표；기증고여림파결전이밀절상관，제시기가능예시궁경암불량예후。령외，량자재궁경암조직중표체증고，가능시궁경암발생발전적원인지일。
Objective:To explore the diagnostic value of the relationship between vascular endothelial growth factorsA and C(VEGF-A and VEGF-C) levels in serum of patients with cervical cancer and lymph node metastasis.Methods:ELISA was used to detect the expression levels of VEGF-A and VEGF-C,in serum of 10 cases with normal cervical tissues,36 cases with cervical intraepithelial neoplasia CINⅢ,33 cases with cervical cancer without lymph node metastasis,10 cases of cervical cancer with lymph node metastasis.The difference of expression levels of VEGF-A and VEGF-C were compared among different groups.Results:The expression level of VEGF-A and VEGF-C in serum in cervical cancer group without lymph node metastasis was significantly higher than those in normal cervical tissues group and CINⅢ group (P<0.05);the expression level of VEGF-A and VEGF-C in serum in cervical cancer group with lymph node metastasis was significantly higher than that in cervical cancer group without lymph node metastasis (P<0.05);There was no significant difference in expression levels of VEGF-A and VEGF-C in serum between CINⅢ group and normal cervical tissues group (P>0.05).Conclusion:The increase of VEGF-A and VEGF-C serum levels in patients with cervical cancer may be related to lymph node metastasis of cervical carcinoma,which may be used as a useful indicator for predicting lymph node metastasis of cervical cancer;In VEGF-A and VEGF-C may indicate adverse prognosis of cervical cancer,the change of serum VEGF-A and VEGF-C level may become an indicator for early diagnosis of cervical cancer.
