CAJ | 학술논문

　　目的探讨非甾体类抗炎药(NSAIDs)对神经胶质瘤细胞增殖的影响。方法选用神经胶质瘤细胞U251，分别加入0μmol/L、10μmol/L、20μmol/L、40μmol/L和80μmol/L的塞来昔布进行处理。四甲基偶氮唑盐比色(MTT)法检测U251细胞增殖水平及抑制率，流式细胞术检测细胞凋亡率。结果随塞来昔布浓度的增加，神经胶质瘤细胞U251增殖水平明显降低，增值抑制率升高。不同浓度和不同作用时间，塞来昔布对U251细胞增殖抑制率有显著性差异(P<0.05)；流式细胞术细胞凋亡率检测显示，80μmol/L塞来昔布处理48 h的U251细胞凋亡率(17.86%)较0μmol/L (11.23%)增高(P<0.05)。结论塞来昔布可抑制人胶质瘤细胞U251的生长和增殖，促进U251的凋亡发生，以80μmol/L为佳。
　　목적탐토비치체류항염약(NSAIDs)대신경효질류세포증식적영향。방법선용신경효질류세포U251，분별가입0μmol/L、10μmol/L、20μmol/L、40μmol/L화80μmol/L적새래석포진행처리。사갑기우담서염비색(MTT)법검측U251세포증식수평급억제솔，류식세포술검측세포조망솔。결과수새래석포농도적증가，신경효질류세포U251증식수평명현강저，증치억제솔승고。불동농도화불동작용시간，새래석포대U251세포증식억제솔유현저성차이(P<0.05)；류식세포술세포조망솔검측현시，80μmol/L새래석포처리48 h적U251세포조망솔(17.86%)교0μmol/L (11.23%)증고(P<0.05)。결론새래석포가억제인효질류세포U251적생장화증식，촉진U251적조망발생，이80μmol/L위가。
Objective To explore the effect of celecoxib on proliferation and apoptosis of the glioma cells. Methods The glioma cell U251 was used and disposed with different densities of celecoxib (0μmol/L, 10μmol/L, 20μmol/L, 40μmol/L and 80μmol/L) for 24 h, 48 h and 72 h. The methyl thiazolyl tetrazolium (MTT) assay was used to detect the tumor cell proliferation and flow cytometry was used to de-tect the tumor cell apoptosis rate. Results The Glioma U251 cells proliferation were significantly decreased with the increase of density of celecoxib in vitro, and there was significant difference in the inhibited rate in different density and different time (P<0.05). The apoptosis rate was higher in the density of 80μmol/L (17.86%) than in that of 0μmol/L (11.23%) (P<0.05). Conclusion Celecoxib can inhabit the pro-liferation of glioma U251 cells, and promote the apoptosis especially with the density of 80μmol/L.