CAJ | 학술논문

　　目的探讨富血小板纤维蛋白对粗糙钛板表面人牙龈成纤维细胞增殖和分化的影响。方法体外培养人牙龈成纤维细胞，传至第4代与粗糙钛板联合培养用于实验。分别在普通培养基和含富血小板纤维蛋白的培养基中培养，MTT、RT-PCR、蛋白印迹技术进行检测。结果 MTT结果显示含加入富血小板纤维蛋白能明显促进粗糙钛板表面人牙龈成纤维细胞的增殖，差异有统计学意义(P＜0.05)。RT-PCR提示两组均有Type(Ⅰ)collagen和bFGF的mRNA表达，含富血小板纤维蛋白组mRNA表达比普通组明显增强。WB提示，Ⅰ型胶原蛋白表达在含富血小板纤维蛋白组中明显增强。结论富血小板纤维蛋白能促进粗糙钛板表面人牙龈成纤维细胞的增殖和分化。
　　목적탐토부혈소판섬유단백대조조태판표면인아간성섬유세포증식화분화적영향。방법체외배양인아간성섬유세포，전지제4대여조조태판연합배양용우실험。분별재보통배양기화함부혈소판섬유단백적배양기중배양，MTT、RT-PCR、단백인적기술진행검측。결과 MTT결과현시함가입부혈소판섬유단백능명현촉진조조태판표면인아간성섬유세포적증식，차이유통계학의의(P＜0.05)。RT-PCR제시량조균유Type(Ⅰ)collagen화bFGF적mRNA표체，함부혈소판섬유단백조mRNA표체비보통조명현증강。WB제시，Ⅰ형효원단백표체재함부혈소판섬유단백조중명현증강。결론부혈소판섬유단백능촉진조조태판표면인아간성섬유세포적증식화분화。
Objective To study the effect of platelet rich fibrin (PRF) on proliferation and differentiation of human gingival fibroblasts on rough titanium surface. Methods Human gingival fibroblasts were cultured in vitro, and their fourth generation was co-cultured with rough titanium. The cells were then cultured in common medium and PRF medium respectively, and detected by MTT, RT-PCR and Western blot respectively. Results MTT showed that PRF could promote the proliferation of human gingival fibroblasts(P < 0.05). RT-PCR demonstrated that the expression level of typeⅠcollagen mRNA was higher than that of bFGF mRNA in PRF medium than in common medium(P<0.05). Western blot displayed that the expression level of typeⅠcollagen was higher in PRF medium than in common medium(P<0.05). Conclusion PRF can accelerate the proliferation and differentiation of human gingival fibroblasts on rough titanium surface.