CAJ | 학술논문

　　目的探讨白细胞介素-18（IL-18）基因启动子区-137G/C多态性及血清IL-18水平与急性脑梗死的相关性。方法用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测急性脑梗死患者153例及健康对照组114例的IL-18基因启动子区-137G/C多态性，用酶联免疫吸附法（ELISA）检测两组血清IL-18浓度。结果与对照组相比，急性脑梗死组IL-18基因启动子区-137G/C多态性各基因型分布及等位基因频率差异均有统计学意义（P＜0．05），携带C等位基因的个体急性脑梗死的相对风险度要比未携带者增加1．672倍。急性脑梗死组血清IL-18浓度明显高于对照组（P＜0．05），急性脑梗死组携带C等位基因的个体的血清IL-18浓度明显高于未携带者（P＜0．05）。结论IL-18基因启动子区-137G/C多态性与急性脑梗死的发病具有相关性，携带C等位基因可促使个体高度表达IL-18，通过影响IL-18血清浓度从而对急性脑梗死的发生发展产生作用，增加急性脑梗死的发病风险。
　　목적탐토백세포개소-18（IL-18）기인계동자구-137G/C다태성급혈청IL-18수평여급성뇌경사적상관성。방법용취합매련반응-한제성편단장도다태성（PCR-RFLP）기술검측급성뇌경사환자153례급건강대조조114례적IL-18기인계동자구-137G/C다태성，용매련면역흡부법（ELISA）검측량조혈청IL-18농도。결과여대조조상비，급성뇌경사조IL-18기인계동자구-137G/C다태성각기인형분포급등위기인빈솔차이균유통계학의의（P＜0．05），휴대C등위기인적개체급성뇌경사적상대풍험도요비미휴대자증가1．672배。급성뇌경사조혈청IL-18농도명현고우대조조（P＜0．05），급성뇌경사조휴대C등위기인적개체적혈청IL-18농도명현고우미휴대자（P＜0．05）。결론IL-18기인계동자구-137G/C다태성여급성뇌경사적발병구유상관성，휴대C등위기인가촉사개체고도표체IL-18，통과영향IL-18혈청농도종이대급성뇌경사적발생발전산생작용，증가급성뇌경사적발병풍험。
Objective To investigate the correlation between polymorphism of promoter region of interleulin -18 (IL-18) gene at position -137G/C and serum IL-18 level with acute cerebral infarction .Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of promoter region of IL-18 gene at position -137G/C in 153 cases of acute cerebral infarction and 114 normal controls,and enzyme-linked immunosorbent assay (ELISA) was used to test serum IL-18 level in the two groups.Results There was significant difference in the genotype distribution and allele frequency of polymorphism of promoter region of IL -18 gene at position-137G/C between acute cerebral infarction group and normal controls (P<0.05).The risk of acute cerebral infarction to the carriers with C allele genetype increased to 1.672 times as compared with the cases without C allele genotype .The serum IL-18 level in the acute cerebral infarction group was significantly higher than the controls (P<0.05),and the carriers with C allele genotype showed significantly higher serum IL -18 levels compared with the cases without C allele genotype in acute cerebral infarction group (P<0.05).Conclusion The polymorphism of promoter region of IL-18 gene at position -137G/C is associated with the onset of acute cerebral infarction .The C allele genotype may lead to a high expression of IL-18,which plays a role on the occurrence and development of acute cerebral infarction by affecting serum IL-18 level,and may increase the risk of acute cerebral infarction .