CAJ | 학술논문

　　目的：探讨萝卜硫素（SF）对白血病细胞化疗药物敏感性的作用。方法：琼脂半固体集落培养法和MTT法检测不同浓度的萝卜硫素对HL-60细胞增殖的影响及协同化疗药对细胞生长的抑制作用；PHA-LCM液相原半固体二步培养SF协同化疗药抑制人原代白血病细胞生长。结果：琼脂半固体集落培养法和MTT法证实在一定浓度范围内SF对HL-60细胞增殖抑制（P<0.05，P<0.01），呈剂量依赖性，SF能够增强HL-60细胞对化疗药物高三尖杉酯碱（Hom）和阿糖胞苷（Ara-c）敏感性（P<0.05）；PHA-LCM液相原半固体二步培养法证实SF在一定浓度范围内对人原代白血病细胞有抑制作用（P<0.05，P<0.01），呈剂量依赖性，并增加人原代白血病细胞对化疗药Hom和Ara-c敏感性。结论：萝卜硫素在一定浓度下有效抑制白血病细胞增殖，并能增强化疗药物的敏感性。
　　목적：탐토라복류소（SF）대백혈병세포화료약물민감성적작용。방법：경지반고체집락배양법화MTT법검측불동농도적라복류소대HL-60세포증식적영향급협동화료약대세포생장적억제작용；PHA-LCM액상원반고체이보배양SF협동화료약억제인원대백혈병세포생장。결과：경지반고체집락배양법화MTT법증실재일정농도범위내SF대HL-60세포증식억제（P<0.05，P<0.01），정제량의뢰성，SF능구증강HL-60세포대화료약물고삼첨삼지감（Hom）화아당포감（Ara-c）민감성（P<0.05）；PHA-LCM액상원반고체이보배양법증실SF재일정농도범위내대인원대백혈병세포유억제작용（P<0.05，P<0.01），정제량의뢰성，병증가인원대백혈병세포대화료약Hom화Ara-c민감성。결론：라복류소재일정농도하유효억제백혈병세포증식，병능증강화료약물적민감성。
Objective: To investigate the effect of sulforaphane (SF) on chemotherapy sensitivity of leukemia cells. Methods: Semi-solid agar colony culture assay and MMT method were used to observe the effect of different con-centrations of SF on HL-60 cell proliferation and homoharringtonine+ ara-c chemotherapy on cell growth. PHA-LCM two-stage culture method was used to detect the effect of SF and homoharringtonine+ara-c chemotherapy on human primary leukemia cells. Results: With a certain range of concentrations, SF inhibited the proliferation of HL-60 cells and the growth of human primary leukemia cells in a dose-dependent manner (P<0.05,P<0.01). SF enhanced homoharringtonine+ara-c chemotherapy sensitivity of HL-60 cells (P<0.05) and human primary leukemia cells. Conclusion: SF can inhibit the proliferation of leukemia cells with a certain range of concentrations and en-hance chemotherapy sensitivity of these cells.